Reference - Detail
|Author||Wu S, Yano S, Chen J, Hisanaga A, Sakao K, He X, He J, Hou DX.|
|Title||Polyphenols from Lonicera caerulea L. Berry Inhibit LPS-Induced Inflammation through Dual Modulation of Inflammatory and Antioxidant Mediators.|
|Journal||J. Agric. Food Chem.|
Lonicera caerulea L. berry polyphenols (LCBP) are considered as major components for bioactivity. This study aimed to clarify the molecular mechanisms by monitoring inflammatory and antioxidant mediator actions in lipopolysaccharide (LPS)-induced mouse paw edema and macrophage cell model. LCBP significantly attenuated LPS-induced paw edema (3.0 ± 0.1 to 2.8 ± 0.1 mm, P < 0.05) and reduced (P < 0.05) serum levels of monocyte chemotactic protein-1 (MCP-1, 100.9 ± 2.3 to 58.3 ± 14.5 ng/mL), interleukin (IL)-10 (1596.1 ± 424.3 to 709.7 ± 65.7 pg/mL), macrophage inflammatory protein (MIP)-1α (1761.9 ± 208.3 to 1369.1 ± 56.4 pg/mL), IL-6 (1262.8 ± 71.7 to 499.0 ± 67.1 pg/mL), IL-4 (93.3 ± 25.7 to 50.7 ± 12.5 pg/mL), IL-12(p-70) (580.4 ± 132.0 to 315.2 ± 35.1 pg/mL), and tumor necrosis factor-α (TNF-α, 2045.5 ± 264.9 to 1270.7 ± 158.6 pg/mL). Cell signaling analysis revealed that LCBP inhibited transforming growth factor β activated kinase-1 (TAK1)-mediated mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) pathways, and enhanced the expression of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and manganese-dependent superoxide dismutase (MnSOD) in earlier response. Moreover, cyanidin 3-glucoside (C3G) and (-)-epicatechin (EC), two major components of LCBP, directly bound to TAK1. These data demonstrated that LCBP might inhibit LPS-induced inflammation by modulating both inflammatory and antioxidant mediators.
|MeSH||Animals Antioxidants / administration & dosage* Antioxidants / chemistry Antioxidants / isolation & purification Chemokine CCL2 / genetics Chemokine CCL2 / immunology Edema / drug therapy* Edema / genetics Edema / immunology Fruit / chemistry Humans Inflammation Mediators / administration & dosage* Inflammation Mediators / isolation & purification Interleukin-12 / genetics Interleukin-12 / immunology Interleukin-4 / genetics Interleukin-4 / immunology Interleukin-6 / genetics Interleukin-6 / immunology Lipopolysaccharides / adverse effects Lipopolysaccharides / immunology Lonicera / chemistry* Male Mice Mice, Inbred ICR Mitogen-Activated Protein Kinases / genetics Mitogen-Activated Protein Kinases / immunology NF-kappa B / genetics NF-kappa B / immunology Plant Extracts / administration & dosage* Plant Extracts / chemistry Plant Extracts / isolation & purification Polyphenols / administration & dosage* Polyphenols / chemistry Polyphenols / isolation & purification|
|Human and Animal Cells||RAW 264(RCB0535)|