論文 - 詳細
| RRC ID | 52249 |
|---|---|
| 著者 | Nashimoto Y, Hayashi T, Kunita I, Nakamasu A, Torisawa YS, Nakayama M, Takigawa-Imamura H, Kotera H, Nishiyama K, Miura T, Yokokawa R. |
| タイトル | Integrating perfusable vascular networks with a three-dimensional tissue in a microfluidic device. |
| ジャーナル | Integr Biol (Camb) |
| Abstract |
Creating vascular networks in tissues is crucial for tissue engineering. Although recent studies have demonstrated the formation of vessel-like structures in a tissue model, long-term culture is still challenging due to the lack of active perfusion in vascular networks. Here, we present a method to create a three-dimensional cellular spheroid with a perfusable vascular network in a microfluidic device. By the definition of the cellular interaction between human lung fibroblasts (hLFs) in a spheroid and human umbilical vein endothelial cells (HUVECs) in microchannels, angiogenic sprouts were induced from microchannels toward the spheroid; the sprouts reached the vessel-like structures in a spheroid to form a continuous lumen. We demonstrated that the vascular network could administer biological substances to the interior of the spheroid. As cell density in the spheroid is similar to that of a tissue, the perfusable vasculature model opens up new possibilities for a long-term tissue culture in vitro. |
| 巻・号 | 9(6) |
| ページ | 506-518 |
| 公開日 | 2017-6-19 |
| DOI | 10.1039/c7ib00024c |
| PMID | 28561127 |
| MeSH | Blood Vessels / cytology Blood Vessels / growth & development* Coculture Techniques Equipment Design Fibroblasts / cytology Fluorescent Dyes Human Umbilical Vein Endothelial Cells Humans Lab-On-A-Chip Devices* Lung / cytology Neovascularization, Physiologic* Perfusion Spheroids, Cellular / cytology Tissue Culture Techniques Tissue Engineering / instrumentation* Tissue Engineering / methods |
| IF | 2.291 |
| 引用数 | 40 |
| リソース情報 | |
| ヒト・動物細胞 | MCF7(RCB1904) |