RRC ID 52262
著者 Nathanael AJ, Oyane A, Nakamura M, Sakamaki I, Nishida E, Kanemoto Y, Miyaji H.
タイトル In Vitro and in Vivo Analysis of Mineralized Collagen-Based Sponges Prepared by a Plasma- and Precursor-Assisted Biomimetic Process.
ジャーナル ACS Appl Mater Interfaces
Abstract Three-dimensional (3D) porous scaffolds for supporting cell adhesion and growth play a vital role in tissue engineering applications. In the present study, three different collagen-based 3D sponges were functionalized by apatite coating. The sponges were coated with apatite on their outer and inner surfaces while retaining their interconnecting pores. To achieve this, we employed a vacuum degassing system in our plasma- and precursor-assisted biomimetic process using a supersaturated calcium phosphate solution. The resulting apatite-coated sponges (mineralized sponges) showed better cell adhesion properties in vitro for osteoblast-like MC3T3-E1 cells compared to that of uncoated sponges. The three mineralized sponges were implanted in the subcutaneous tissue of rats. Upon histological evaluation after 10 days, the mineralized sponges showed cell in-growth rates that were approximately 4-fold greater than those of the untreated sponges without any notable inflammatory reactions. As these sponges are composed of clinically approved collagen-based frameworks and possess a 3D porous structure with a mineralized surface appropriate for cell adhesion and internalization, further in vitro and in vivo studies should be conducted regarding tissue engineering applications.
巻・号 9(27)
ページ 22185-22194
公開日 2017-7-12
DOI 10.1021/acsami.7b04776
PMID 28621514
MeSH Animals Apatites Biomimetics* Collagen Osteoblasts Rats Tissue Engineering Tissue Scaffolds
IF 8.758
引用数 7
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)