RRC ID 52269
著者 Nagao M, Tanabe N, Manaka S, Takayama T, Kawato T, Torigoe G, Sekino J, Tsukune N, Ozaki M, Maeno M, Suzuki N, Sato S.
タイトル Low-intensity pulsed ultrasound inhibits lipopolysaccharide-induced IL-6 and RANKL expression in osteoblasts.
ジャーナル J Oral Sci
Abstract Periodontal disease is caused by inflammation induced by Porphyromonas gingivalis (P.g.) lipopolysaccharide (LPS) and involves expression of proinflammatory cytokines such as interleukin (IL)-1, IL-6, tumor necrosis factor-α, and receptor activator of nuclear factor kappa B ligand (RANKL), which are implicated in bone resorption. Low-intensity pulsed ultrasound (LIPUS) is commonly used in the treatment of bone fracture. However, the mechanisms by which LIPUS inhibits LPS-induced inflammatory cytokines are poorly understood. Therefore, we investigated the effects of LIPUS on LPS-induced expression of the proinflammatory cytokines IL-6 and RANKL. MC3T3-E1 cells were incubated in the presence or absence of P.g. LPS and then stimulated with LIPUS for 30 min/day for a maximum of 14 days. LPS increased mRNA and protein expressions of IL-6 and RANKL on day 14. In addition, mRNA expression of COX-2 LPS was higher after 3 and 7 days of LIPUS treatment. PGE2 was induced by LPS after 7 and 14 days of culture. LIPUS suppressed all stimulatory effects of LPS. These results suggest that LIPUS inhibits LPS-induced expression of inflammation cytokines by suppressing PGE2 production and might thus have potential applications in the treatment of periodontitis.
巻・号 59(2)
ページ 303-309
公開日 2017-1-1
DOI 10.2334/josnusd.16-0624
PMID 28637991
MeSH 3T3 Cells Animals Enzyme-Linked Immunosorbent Assay Interleukin-6 / metabolism* Lipopolysaccharides / pharmacology* Mice Osteoblasts / drug effects* Osteoblasts / metabolism RANK Ligand / metabolism* Real-Time Polymerase Chain Reaction Ultrasonics*
IF 1.2
引用数 9
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)