論文 - 詳細
| RRC ID | 52269 |
|---|---|
| 著者 | Nagao M, Tanabe N, Manaka S, Takayama T, Kawato T, Torigoe G, Sekino J, Tsukune N, Ozaki M, Maeno M, Suzuki N, Sato S. |
| タイトル | Low-intensity pulsed ultrasound inhibits lipopolysaccharide-induced IL-6 and RANKL expression in osteoblasts. |
| ジャーナル | J Oral Sci |
| Abstract |
Periodontal disease is caused by inflammation induced by Porphyromonas gingivalis (P.g.) lipopolysaccharide (LPS) and involves expression of proinflammatory cytokines such as interleukin (IL)-1, IL-6, tumor necrosis factor-α, and receptor activator of nuclear factor kappa B ligand (RANKL), which are implicated in bone resorption. Low-intensity pulsed ultrasound (LIPUS) is commonly used in the treatment of bone fracture. However, the mechanisms by which LIPUS inhibits LPS-induced inflammatory cytokines are poorly understood. Therefore, we investigated the effects of LIPUS on LPS-induced expression of the proinflammatory cytokines IL-6 and RANKL. MC3T3-E1 cells were incubated in the presence or absence of P.g. LPS and then stimulated with LIPUS for 30 min/day for a maximum of 14 days. LPS increased mRNA and protein expressions of IL-6 and RANKL on day 14. In addition, mRNA expression of COX-2 LPS was higher after 3 and 7 days of LIPUS treatment. PGE2 was induced by LPS after 7 and 14 days of culture. LIPUS suppressed all stimulatory effects of LPS. These results suggest that LIPUS inhibits LPS-induced expression of inflammation cytokines by suppressing PGE2 production and might thus have potential applications in the treatment of periodontitis. |
| 巻・号 | 59(2) |
| ページ | 303-309 |
| 公開日 | 2017-1-1 |
| DOI | 10.2334/josnusd.16-0624 |
| PMID | 28637991 |
| MeSH | 3T3 Cells Animals Enzyme-Linked Immunosorbent Assay Interleukin-6 / metabolism* Lipopolysaccharides / pharmacology* Mice Osteoblasts / drug effects* Osteoblasts / metabolism RANK Ligand / metabolism* Real-Time Polymerase Chain Reaction Ultrasonics* |
| IF | 1.2 |
| 引用数 | 9 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |