RRC ID |
52269
|
著者 |
Nagao M, Tanabe N, Manaka S, Takayama T, Kawato T, Torigoe G, Sekino J, Tsukune N, Ozaki M, Maeno M, Suzuki N, Sato S.
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タイトル |
Low-intensity pulsed ultrasound inhibits lipopolysaccharide-induced IL-6 and RANKL expression in osteoblasts.
|
ジャーナル |
J Oral Sci
|
Abstract |
Periodontal disease is caused by inflammation induced by Porphyromonas gingivalis (P.g.) lipopolysaccharide (LPS) and involves expression of proinflammatory cytokines such as interleukin (IL)-1, IL-6, tumor necrosis factor-α, and receptor activator of nuclear factor kappa B ligand (RANKL), which are implicated in bone resorption. Low-intensity pulsed ultrasound (LIPUS) is commonly used in the treatment of bone fracture. However, the mechanisms by which LIPUS inhibits LPS-induced inflammatory cytokines are poorly understood. Therefore, we investigated the effects of LIPUS on LPS-induced expression of the proinflammatory cytokines IL-6 and RANKL. MC3T3-E1 cells were incubated in the presence or absence of P.g. LPS and then stimulated with LIPUS for 30 min/day for a maximum of 14 days. LPS increased mRNA and protein expressions of IL-6 and RANKL on day 14. In addition, mRNA expression of COX-2 LPS was higher after 3 and 7 days of LIPUS treatment. PGE2 was induced by LPS after 7 and 14 days of culture. LIPUS suppressed all stimulatory effects of LPS. These results suggest that LIPUS inhibits LPS-induced expression of inflammation cytokines by suppressing PGE2 production and might thus have potential applications in the treatment of periodontitis.
|
巻・号 |
59(2)
|
ページ |
303-309
|
公開日 |
2017-1-1
|
DOI |
10.2334/josnusd.16-0624
|
PMID |
28637991
|
MeSH |
3T3 Cells
Animals
Enzyme-Linked Immunosorbent Assay
Interleukin-6 / metabolism*
Lipopolysaccharides / pharmacology*
Mice
Osteoblasts / drug effects*
Osteoblasts / metabolism
RANK Ligand / metabolism*
Real-Time Polymerase Chain Reaction
Ultrasonics*
|
IF |
1.2
|
引用数 |
9
|
リソース情報 |
ヒト・動物細胞 |
MC3T3-E1(RCB1126) |