RRC ID 52270
Author Takamura T, Suguro H, Mikami Y, Iwase T, Komiyama Y, Kuyama K, Komiyama K, Oki H.
Title Comparison of gene expression profiles of gingival carcinoma Ca9-22 cells and colorectal adenocarcinoma HT-29 cells to identify potentially important mediators of SLPI-induced cell migration.
Journal J Oral Sci
Abstract Secretory leukocyte protease inhibitor (SLPI) is a serine protease inhibitor whose expression level is positively correlated with tumor aggressiveness and metastatic potential. However, the mechanism underlying SLPI-induced enhancement of malignant phenotype is not completely understood. The malignancy of cancer cells is highly dependent on cell migration activity. Our previous study revealed that gingival carcinoma Ca9-22 cells, but not colorectal adenocarcinoma HT-29 cells, expressed SLPI. Therefore, we investigated the migration activity of these two cell types to understand the nature of SLPI-mediated tumor aggressiveness and metastatic potential. In vitro wound healing assay indicated that HT-29 cells and SLPI-deleted Ca9-22 cells showed lower migration activity than wild-type Ca9-22 cells, suggesting that SLPI-induced cell migration plays an important role in tumor aggressiveness and metastatic potential. In addition, our gene expression profiling study based on microarray data for the three cell types identified a number of candidates, including LCP1 and GLI, that could be key molecules in the mechanism of SLPI-induced cell migration.
Volume 59(2)
Pages 279-287
Published 2017-1-1
DOI 10.2334/josnusd.16-0534
PMID 28637988
MeSH Adenocarcinoma / genetics* Adenocarcinoma / pathology Cell Movement / physiology* Colorectal Neoplasms / genetics* Colorectal Neoplasms / pathology Gene Expression Profiling* Gingival Neoplasms / genetics* Gingival Neoplasms / pathology HT29 Cells Humans Neoplasm Metastasis Secretory Leukocyte Peptidase Inhibitor / genetics Secretory Leukocyte Peptidase Inhibitor / physiology*
IF 1.2
Times Cited 0
Human and Animal Cells Ca9-22(RCB1976)