RRC ID 5229
著者 Song L, Dong X.
タイトル Clostridium amylolyticum sp. nov., isolated from H2-producing UASB granules.
ジャーナル Int J Syst Evol Microbiol
Abstract A Gram-stain-positive, strictly anaerobic, mesophilic, amylolytic, rod-shaped bacterium, designated strain SW408(T), was isolated from a laboratory-scale H(2)-producing upflow anaerobic sludge blanket reactor. The strain grew at 24-45 degrees C (no growth at or below 22 degrees C or at or above 47 degrees C), with optimum growth at 37 degrees C. The pH range for growth was 4.0-9.0 (no growth at or below pH 3.6 or at or above pH 9.3), with optimum growth at pH 7.0. Starch, cellobiose, glucose, fructose, galactose, lactose, maltose, mannose, ribose and sucrose supported growth. The major end products from glucose fermentation were ethanol, acetate, hydrogen and carbon dioxide. Abundant H(2) was produced from starch fermentation. The DNA G+C content was 33.1 mol% (T(m) method). Phylogenetic analysis based on 16S rRNA gene sequence analysis showed that the bacterium represents a previously unrecognized species within Clostridium rRNA cluster I and is most closely related to the type strain of Clostridium frigidicarnis (94.9% similarity). On the basis of phenotypic, genotypic and phylogenetic characteristics, strain SW408(T) was identified as a representative of a novel species of the genus Clostridium, for which the name Clostridium amylolyticum sp. nov. is proposed. The type strain is SW408(T) (=JCM 14823(T)=AS 1.5069(T)=CGMCC 1.5069(T)).
巻・号 58(Pt 9)
ページ 2132-5
公開日 2008-9-1
DOI 10.1099/ijs.0.65635-0
PII 58/9/2132
PMID 18768618
MeSH Acetates / metabolism Amylopectin / metabolism Anaerobiosis Bacterial Typing Techniques Base Composition Carbohydrate Metabolism Carbon Dioxide / metabolism Clostridium / classification* Clostridium / genetics Clostridium / isolation & purification* Clostridium / physiology DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Ethanol / metabolism Genes, rRNA Hydrogen / metabolism Hydrogen-Ion Concentration Molecular Sequence Data Phylogeny RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid Sewage / microbiology* Temperature
IF 2.415
引用数 11
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM14823