論文 - 詳細
| RRC ID | 52297 |
|---|---|
| 著者 | Bamba Y, Kanemura Y, Okano H, Yamasaki M. |
| タイトル | Visualization of migration of human cortical neurons generated from induced pluripotent stem cells. |
| ジャーナル | J Neurosci Methods |
| Abstract |
BACKGROUND:Neuronal migration is considered a key process in human brain development. However, direct observation of migrating human cortical neurons in the fetal brain is accompanied by ethical concerns and is a major obstacle in investigating human cortical neuronal migration. NEW METHOD:We established a novel system that enables direct visualization of migrating cortical neurons generated from human induced pluripotent stem cells (hiPSCs). RESULTS:We observed the migration of cortical neurons generated from hiPSCs derived from a control and from a patient with lissencephaly. METHODS:Our system needs no viable brain tissue, which is usually used in slice culture. Migratory behavior of human cortical neuron can be observed more easily and more vividly by its fluorescence and glial scaffold than that by earlier methods. CONCLUSIONS:Our in vitro experimental system provides a new platform for investigating development of the human central nervous system and brain malformation. |
| 巻・号 | 289 |
| ページ | 57-63 |
| 公開日 | 2017-9-1 |
| DOI | 10.1016/j.jneumeth.2017.07.004 |
| PII | S0165-0270(17)30240-6 |
| PMID | 28694214 |
| MeSH | Cell Culture Techniques* Cell Line Cell Movement / physiology* Cerebral Cortex / cytology Cerebral Cortex / physiology* Ependymoglial Cells / cytology Ependymoglial Cells / physiology Humans Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / physiology* Lissencephaly / physiopathology Luminescent Proteins / genetics Luminescent Proteins / metabolism Neurons / cytology Neurons / physiology* Optical Imaging / methods* Transfection |
| IF | 2.785 |
| 引用数 | 7 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) 409B2(HPS0076) |