Reference - Detail
RRC ID | 52306 |
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Author | Sangkanjanavanich N, Kawai M, Kakuda T, Takai S. |
Title | Rescue of an intracellular avirulent Rhodococcus equi replication defect by the extracellular addition of virulence-associated protein A. |
Journal | J Vet Med Sci |
Abstract |
Rhodococcus equi is a facultative intracellular bacterium that can escape from bactericidal mechanisms associated with phagocytosis. Virulence-associated protein A (VapA), encoded on a virulence-associated plasmid, is essential for intracellular survival in macrophages, but its function is not known. Here, we show that the extracellular addition of recombinant glutathione S-transferase (GST)-VapA fusion protein rescued the intracellular replication defect of a mutant lacking the vapA gene. Furthermore, the virulence-plasmid-cured strain could also multiply to nearly wild-type levels by the addition of GST-VapA. The present data suggest that VapA can alter the intraphagocytic environment, thereby affecting its suitability for the growth of R. equi. |
Volume | 79(8) |
Pages | 1323-1326 |
Published | 2017-8-4 |
DOI | 10.1292/jvms.17-0350 |
PMID | 28690290 |
PMC | PMC5573816 |
MeSH | Actinomycetales Infections / microbiology Animals Cell Line Gene Deletion Genes, Bacterial / genetics Glutathione Transferase Macrophages / microbiology Mice Plasmids Recombinant Proteins Rhodococcus equi / genetics* Rhodococcus equi / growth & development Rhodococcus equi / pathogenicity* Staphylococcal Protein A / genetics* Virulence / genetics* Virulence Factors* |
IF | 0.803 |
Times Cited | 2 |
Resource | |
Human and Animal Cells | J774.1(RCB0434) |