| RRC ID |
52317
|
| Author |
Rodriguez R, Yoshimura K, Shibata Y, Miyamoto Y, Tanaka R, Uyama R, Sasa K, Suzuki D, Miyazaki T, Kamijo R.
|
| Title |
Nanoindentation time-dependent deformation/recovery suggestive of methylglyoxal induced glycation in calcified nodules.
|
| Journal |
Nanomedicine
|
| Abstract |
Although empirical findings have indicated increase in bone fracture risk in type 2 diabetes patients, that has yet to be proven by results obtained at the material level. Here, we report evidence showing nanoscale time-dependent deformation/recovery of in vitro calcified nodules mimicking bone turnover in type 2 diabetes in respect to methylglyoxal (MG)-induced glycation. Nanoindentation test results revealed that calcified nodules cultured with MG did not show adequate dimensional recovery, despite a large creep rate during constant load indentation testing. This lesser recovery is likely based on the linear matrix polymerization network formed by advanced glycation end products (AGEs) as a secondary product of MG. Since elevated serum MG and abnormal bone turnover related to the amount of AGEs are observed in cases of type 2 diabetes, this time-dependent behavior may be one of the factors of the bone fracture mechanism at the material level in affected patients.
|
| Volume |
13(8)
|
| Pages |
2545-2553
|
| Published |
2017-11-1
|
| DOI |
10.1016/j.nano.2017.07.003
|
| PII |
S1549-9634(17)30133-8
|
| PMID |
28754466
|
| MeSH |
Bone and Bones / metabolism
Bone and Bones / pathology
Calcinosis / metabolism*
Calcinosis / pathology
Cell Line
Cell Proliferation
Diabetes Mellitus, Type 2 / metabolism*
Diabetes Mellitus, Type 2 / pathology
Glycation End Products, Advanced / metabolism*
Humans
Osteoblasts / cytology
Osteoblasts / metabolism*
Osteoblasts / pathology
Pyruvaldehyde / metabolism*
|
| IF |
4.3
|
| Times Cited |
2
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126) |
