論文 - 詳細
| RRC ID | 52323 |
|---|---|
| 著者 | Chen Z, Wu Y, Ma YS, Kobayashi Y, Zhao YY, Miura Y, Chiba H, Hui SP. |
| タイトル | Profiling of cardiolipins and their hydroperoxides in HepG2 cells by LC/MS. |
| ジャーナル | Anal Bioanal Chem |
| Abstract |
Cardiolipin (CL) exists as crucial functional phospholipid in mitochondria. The oxidation of CL is concerned with mitochondrial dysfunction and various diseases. As main oxidation products, CL hydroperoxide (CL-OOH) plays a key role in intermediating oxidative reaction. Thus, direct analysis of CL-OOH is of great interest. In the present study, CL and CL-OOH profiles were analyzed in oxidized HepG2 cell lipid via HPLC-Orbitrap MS/MS. Furthermore, the contents of individual molecular species were compared between intact and AAPH-oxidized HepG2 cells. In total, 46 CL and 18 CL-OOH were identified from oxidized cell lipids, while 21 CL and 9 CL-OOH were detected in AAPH-treated cells. Most CL depleted significantly after AAPH inducement, with percentages varying from 8.3% (CL70:7) to 73.7% (CL72:4), depending on fatty acyl composition. While almost all the CL-OOH remarkably increased, among them 68:6-, 72:6-, and 72:7-OOHs were only detected in AAPH-treated cells. CL68:5- and CL68:4-OOH were the most abundant species, while CL70:5-OOH among all the species expressed the highest oxidation percentage of the corresponding CL. Our results showed practical separation, identification, and semi-quantitation of CL-OOH species, which could contribute to approaches to lipidomic analysis of CL and CL-OOH, as well as tracing biomarkers in mitochondrial oxidative stress diagnosis. Graphical abstract Illustration represents cardiolipin hydroperoxide structure and its content increasing in AAPH-treated HepG2 cells by LC/MS analysis. |
| 巻・号 | 409(24) |
| ページ | 5735-5745 |
| 公開日 | 2017-9-1 |
| DOI | 10.1007/s00216-017-0515-3 |
| PII | 10.1007/s00216-017-0515-3 |
| PMID | 28762068 |
| MeSH | Cardiolipins / analysis* Chromatography, High Pressure Liquid Hep G2 Cells Hepatocytes / chemistry* Humans Lipid Peroxidation Mitochondria / chemistry Peroxides / analysis* Tandem Mass Spectrometry |
| IF | 3.637 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB1886) |