RRC ID 52339
著者 Hoffmann M, Gogola J, Kotula-Balak M, Ptak A.
タイトル Stimulation of ovarian cell proliferation by tetrabromobisphenol A but not tetrachlorobisphenol A through G protein-coupled receptor 30.
ジャーナル Toxicol In Vitro
Abstract Tetrabromobisphenol A (TBBPA) and tetrachlorobisphenol A (TCBPA) are bisphenol A (BPA) analogs, where the phenolic moieties are substituted with halogens (Br or Cl). Previous studies indicate that BPA has significant proliferative effects on in vitro cultured epithelial ovarian cancer (EOC) cells. Considering this, we analyzed the effects of both TBBPA and TCBPA at 1, 10, and 50nM on ovarian cancer cell proliferation. The majority of malignant ovarian tumors are epithelial in origin, but approximately 10% are classified as ovarian sex cord tumors, with the most common type being granulosa cell tumors (GCTs). OVCAR-3 and KGN cells were used as in vitro models to represent EOCs and GCTs, respectively. Here, we found that TBBPA, but not TCBPA, stimulated OVCAR-3 and KGN cell proliferation, with lower potency than BPA. The stimulatory effects of TBBPA and BPA on cell proliferation were reversed by pre-treatment with a G protein-coupled receptor 30 (GPR30) antagonist in both cell lines, which possess similar basal GPR30 expression levels. Taken together, our results show for the first time that TBBPA, which has lower potency than BPA, stimulates ovarian cancer cell proliferation through the GPR30 pathway.
巻・号 45(Pt 1)
ページ 54-59
公開日 2017-12-1
DOI 10.1016/j.tiv.2017.08.009
PII S0887-2333(17)30225-4
PMID 28811233
MeSH Benzhydryl Compounds / toxicity Benzodioxoles Cell Line, Tumor Cell Proliferation / drug effects Chlorophenols / toxicity* Environmental Pollutants / toxicity Female Flame Retardants / toxicity Gene Expression Regulation / drug effects Humans Ovarian Neoplasms* Phenols / toxicity Polybrominated Biphenyls / toxicity* Quinolines Receptors, Estrogen / genetics Receptors, Estrogen / metabolism* Receptors, G-Protein-Coupled / genetics Receptors, G-Protein-Coupled / metabolism*
IF 2.959
引用数 6
リソース情報
ヒト・動物細胞 KGN(RCB1154)