論文 - 詳細
| RRC ID | 52371 |
|---|---|
| 著者 | Chen P, Miyake M, Tsukamoto M, Tsutsumi Y, Hanawa T. |
| タイトル | Response of preosteoblasts to titanium with periodic micro/nanometer scale grooves produced by femtosecond laser irradiation. |
| ジャーナル | J Biomed Mater Res A |
| Abstract |
To investigate the cellular response to designed topography in vitro, we studied the adhesion, proliferation, osteogenic differentiation, and calcification of mouse preosteoblasts (MC3T3-E1) cultured on titanium (Ti) surfaces with periodic micrometer scale grooves containing nanometer scale ripples in the vertical direction fabricated by single-shot, femtosecond laser irradiation (fsTi). The surface composition and chemical state of fsTi were almost the same as those of mirror-polished Ti without femtosecond laser irradiation (mTi). Cells cultured on fsTi were highly aligned, whereas the cell proliferation rate on fsTi was less than that on mTi. Higher gene expressions of Spp1 and Bglap1 were detected in cells cultured on fsTi than those on mTi, indicating that the periodic micro/nanometer scale grooves topography promoted osteogenic differentiation and calcification. This initial activation of osteoinduction on fsTi generated calcified deposits that were thicker and larger than those on mTi and hence, osteoconductivity was promoted on fsTi. Our findings indicate that femtosecond laser irradiation is a technique with potential for controlling biomaterial-cell interfaces and, in particular, the promotion of osseointegration of Ti. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3456-3464, 2017. |
| 巻・号 | 105(12) |
| ページ | 3456-3464 |
| 公開日 | 2017-12-1 |
| DOI | 10.1002/jbm.a.36202 |
| PMID | 28879673 |
| MeSH | Animals Biocompatible Materials / chemistry* Calcification, Physiologic Cell Adhesion Cell Differentiation Cell Line Cell Proliferation Lasers Mice Nanostructures / chemistry* Osseointegration Osteoblasts / cytology* Osteoblasts / metabolism Osteogenesis Surface Properties Titanium / chemistry* |
| IF | 3.525 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |