論文 - 詳細
| RRC ID | 52381 |
|---|---|
| 著者 | Hirashima T, Hoshuyama M, Adachi T. |
| タイトル | In vitro tubulogenesis of Madin-Darby canine kidney (MDCK) spheroids occurs depending on constituent cell number and scaffold gel concentration. |
| ジャーナル | J Theor Biol |
| Abstract |
In vitro tubulogenesis has been employed as an experimental model system to study tissue morphogenesis of internal organs. It has been previously shown that Madin-Darby canine kidney (MDCK) cells form tubes in the presence of hepatocyte growth factor in 3D cultures. Although these cells are expected to form tube structures in some microenvironments independent of chemical stimulation, little is known about the cellular mechanisms in organizing such an anisotropic multicellular structure. Here, we report 3D culture conditions that induce MDCK tubulogenesis without growth factor stimulation. We found that the cells spontaneously form elongated tube structures through aggregation processes in a specific range of both constituent cell number and scaffold gel concentration, while they form spherical aggregates in other conditions. We then examined cellular activities affecting tubulogenesis and showed that cell proliferation is not required for the tube elongation. Furthermore, we revealed that cells in the tube tips generate traction forces and pull the surrounding scaffold gel to migrate, resulting in the tube elongation. Our results suggest that the constituent cells during the aggregation process interact each other via mechanical forces transmitted in the scaffold gel, leading to the spontaneous tube formation. |
| 巻・号 | 435 |
| ページ | 110-115 |
| 公開日 | 2017-12-21 |
| DOI | 10.1016/j.jtbi.2017.09.009 |
| PII | S0022-5193(17)30427-7 |
| PMID | 28923372 |
| MeSH | Animals Biomechanical Phenomena Cell Aggregation* Cell Count Dogs Gels Kidney Tubules / cytology Kidney Tubules / growth & development* Madin Darby Canine Kidney Cells Morphogenesis* Spheroids, Cellular / cytology |
| IF | 1.833 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MDCK(RCB0995) |