| RRC ID |
52389
|
| 著者 |
Sakata M, Tani H, Anraku M, Kataoka M, Nagata N, Seki F, Tahara M, Otsuki N, Okamoto K, Takeda M, Mori Y.
|
| タイトル |
Analysis of VSV pseudotype virus infection mediated by rubella virus envelope proteins.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Rubella virus (RV) generally causes a systemic infection in humans. Viral cell tropism is a key determinant of viral pathogenesis, but the tropism of RV is currently poorly understood. We analyzed various human cell lines and determined that RV only establishes an infection efficiently in particular non-immune cell lines. To establish an infection the host cells must be susceptible and permissible. To assess the susceptibility of individual cell lines, we generated a pseudotype vesicular stomatitis virus bearing RV envelope proteins (VSV-RV/CE2E1). VSV-RV/CE2E1 entered cells in an RV envelope protein-dependent manner, and thus the infection was neutralized completely by an RV-specific antibody. The infection was Ca2+-dependent and inhibited by endosomal acidification inhibitors, further confirming the dependency on RV envelope proteins for the VSV-RV/CE2E1 infection. Human non-immune cell lines were mostly susceptible to VSV-RV/CE2E1, while immune cell lines were much less susceptible than non-immune cell lines. However, susceptibility of immune cells to VSV-RV/CE2E1 was increased upon stimulation of these cells. Our data therefore suggest that immune cells are generally less susceptible to RV infection than non-immune cells, but the susceptibility of immune cells is enhanced upon stimulation.
|
| 巻・号 |
7(1)
|
| ページ |
11607
|
| 公開日 |
2017-9-14
|
| DOI |
10.1038/s41598-017-10865-2
|
| PII |
10.1038/s41598-017-10865-2
|
| PMID |
28912595
|
| PMC |
PMC5599607
|
| MeSH |
Animals
Cell Line
Coinfection
Genes, Reporter
Genetic Engineering
Humans
Macrophages / immunology
Macrophages / metabolism
Macrophages / virology
Neutralization Tests
Rubella virus / physiology*
Vesicular stomatitis Indiana virus / physiology*
Viral Envelope Proteins / genetics
Viral Envelope Proteins / metabolism*
Viral Tropism
|
| IF |
3.998
|
| 引用数 |
2
|
| リソース情報 |
| ヒト・動物細胞 |
HSQ-89(RCB0789) |
