RRC ID 52408
著者 Terada K, Matsushima Y, Matsunaga K, Takata J, Karube Y, Ishige A, Chiba K.
タイトル The Kampo medicine Yokukansan (YKS) enhances nerve growth factor (NGF)-induced neurite outgrowth in PC12 cells.
ジャーナル Bosn J Basic Med Sci
Abstract Accumulating evidence indicates that neurotrophic factor-like substances involved in the induction of neurotrophic factor synthesis may aid in the treatment of neurological disorders, such as Alzheimer's disease. Yokukansan (YKS), a traditional Kampo medicine, has been used for the treatment of anxiety and mood disorders. In the present study, we aimed to identify the signaling pathways associated with YKS-mediated enhancement of nerve growth factor (NGF)-induced neurite extension in rat pheochromocytoma (PC12) cells. Akt and extracellular-regulated kinase 1/2 (ERK1/2) phosphorylation levels were assessed by western blot analysis, in the presence of YKS and following the treatment with TrkA inhibitor, K252a. YKS treatment (NGF+YKS 0.5 group) enhanced NGF-induced neurite outgrowth and phosphorylation/activation of Akt and ERK1/2 in PC12 cells. Moreover, YKS-induced effects were inhibited by the treatment with the TrkA receptor antagonist K252a (NGF+YKS 0.5+K252a group); no significant difference in neurite outgrowth was observed between K252a-treated (NGF+YKS 0.5+K252a group) and NGF-K252a-treated cells (NGF+K252a group). However, neurite outgrowth in K252a-treated cells (NGF+K252a and NGF+YKS 0.5+K252a group) reached only one-third of the level in NGF-treated cells (NGF group). NGF-mediated Akt phosphorylation increased by YKS was also inhibited by K252a treatment (NGF+YKS 0.5+K252a group), but no significant difference in ERK1/2 phosphorylation was observed between NGF-YKS-K252a- and NGF-treated cells (NGF group). Our results indicate that YKS treatment enhanced NGF-induced neurite outgrowth via induction of Akt and ERK1/2 phosphorylation, following the binding of NGF to the TrkA receptor. These findings may be useful in the development of novel therapeutic strategies for the treatment of Alzheimer's disease.
巻・号 18(3)
ページ 224-233
公開日 2018-8-1
DOI 10.17305/bjbms.2017.2248
PMID 28961087
PMC PMC6087561
MeSH Alzheimer Disease / therapy Animals Cell Differentiation Cell Survival Dose-Response Relationship, Drug Drugs, Chinese Herbal / chemistry Drugs, Chinese Herbal / pharmacology* Medicine, Kampo Mice Nerve Growth Factor / metabolism* Neurites / metabolism* Neuronal Outgrowth / drug effects* PC12 Cells Phosphorylation Rats Receptor, trkA / antagonists & inhibitors Receptor, trkA / metabolism Signal Transduction
IF 1.432
引用数 3
リソース情報
ヒト・動物細胞 PC-12(RCB0009)