論文 - 詳細
| RRC ID | 52411 |
|---|---|
| 著者 | Seike S, Takehara M, Takagishi T, Miyamoto K, Kobayashi K, Nagahama M. |
| タイトル | Delta-toxin from Clostridium perfringens perturbs intestinal epithelial barrier function in Caco-2 cell monolayers. |
| ジャーナル | Biochim Biophys Acta Biomembr |
| Abstract |
Clostridium perfringens delta-toxin is a β-barrel-pore-forming toxin (β-PFT) and a presumptive virulence factor of type B and C strains, which are causative organisms of fatal intestinal diseases in animals. We showed previously that delta-toxin causes cytotoxicity via necrosis in sensitive cells. Here, we examined the effect of delta-toxin on intestinal membrane integrity. Delta-toxin led to a reduction in transepithelial electrical resistance (TEER) and increased the permeability of fluorescence isothiocyanate-conjugated dextran in human intestinal epithelial Caco-2 cells without changing the tight junction proteins, such as zonula occludens-1 (ZO-1), occludin, and claudin-1. On the other hand, delta-toxin reduced the cellular levels of adherence junction protein E-cadherin before cell injury. A disintegrin and metalloprotease (ADAM) 10 facilitates E-cadherin cleavage and was identified as the cellular receptor for alpha-toxin, a β-PFT produced by Staphylococcus aureus. ADAM10 inhibitor (GI254023X) blocked the toxin-induced decrease in TEER and cleavage of E-cadherin. Delta-toxin enhanced ADAM10 activity in a dose- and time-dependent manner. Furthermore, delta-toxin colocalized with ADAM10. These results indicated that ADAM10 plays a key role in delta-toxin-induced intestinal injury. |
| 巻・号 | 1860(2) |
| ページ | 428-433 |
| 公開日 | 2018-2-1 |
| DOI | 10.1016/j.bbamem.2017.10.003 |
| PII | S0005-2736(17)30313-9 |
| PMID | 28988777 |
| MeSH | ADAM10 Protein / metabolism Bacterial Toxins / pharmacology* Caco-2 Cells Cadherins / metabolism Cell Membrane Permeability / drug effects* Claudin-1 / metabolism Dose-Response Relationship, Drug Epithelial Cells / drug effects* Epithelial Cells / metabolism Humans Immunoblotting Intestinal Mucosa / drug effects* Intestinal Mucosa / metabolism Occludin / metabolism Time Factors Zonula Occludens-1 Protein / metabolism |
| IF | 3.79 |
| 引用数 | 6 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |