RRC ID 52412
著者 Osawa Y, Miyamoto T, Ohno S, Ohno E.
タイトル Morphological Analysis of Live Undifferentiated Cells Derived from Induced Pluripotent Stem Cells.
ジャーナル Stem Cells Dev
Abstract Induced pluripotent stem (iPS) cells possess pluripotency and self-renewal ability. Therefore, iPS cells are expected to be useful in regenerative medicine. However, iPS cells form malignant immature teratomas after transplantation into animals, even after differentiation induction. It has been suggested that undifferentiated cells expressing Nanog that remain after differentiation induction are responsible for teratoma formation. Various methods of removing these undifferentiated cells have therefore been investigated, but few methods involve morphological approaches, which may induce less cell damage. In addition, for cells derived from iPS cells to be applied in regenerative medicine, they must be alive. However, detailed morphological analysis of live undifferentiated cells has not been performed. For the above reasons, we assessed the morphological features of live undifferentiated cells remaining after differentiation induction as a basic investigation into the clinical application of iPS cells. As a result, live undifferentiated cells remaining after differentiation induction exhibited a round or oval cytoplasm about 12 μm in diameter and a nucleus. They exhibited nucleo-cytoplasmic (N/C) ratio of about 60% and eccentric nuclei, and they possessed partially granule-like structures in the cytoplasm and prominent nucleoli. Although they were similar to iPS cells, they were smaller than live iPS cells. Furthermore, very small cells were present among undifferentiated cells after differentiation induction. These results suggest that the removal of undifferentiated cells may be possible using the morphological features of live iPS cells and undifferentiated cells after differentiation induction. In addition, this study supports safe regenerative medicine using iPS cells.
巻・号 27(1)
ページ 1-9
公開日 2018-1-1
DOI 10.1089/scd.2017.0112
PMID 28978257
MeSH Animals Cell Differentiation / physiology* Cell Nucleus / physiology Cytoplasm / physiology Induced Pluripotent Stem Cells / cytology* Mice Regenerative Medicine / methods Teratoma / pathology
IF 3.153
引用数 1
リソース情報
ヒト・動物細胞 iPS-MEF-Ng-20D-17(APS0001)