RRC ID |
52414
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Author |
Miyamoto H, Tsujimoto H, Horii T, Ozamoto Y, Ueda J, Takagi T, Saitoh N, Hagiwara A.
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Title |
The influences of a novel anti-adhesion device, thermally cross-linked gelatin film on peritoneal dissemination of tumor cells: The in vitro and in vivo experiments using murine carcinomatous peritonitis models.
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Journal |
J Biomed Mater Res B Appl Biomater
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Abstract |
To create anti-adhesive materials to be more effective and safer, we developed a thermally cross-linked gelatin film that showed superior anti-adhesive effects with excellent peritoneal regeneration. However, it may act as a convenient scaffold for tumor cell growth, thereby accelerating peritoneal dissemination when used in surgery for abdominal tumors. In this study, we tried to clarify this issue using mouse carcinomatous peritonitis models. First, we examined the in vitro tumor cell growth of mouse B16 melanoma or Colon26 cells on the gelatin film or the conventional hyarulonate/carboxymethylcellulose film. Tumor cell growth on each film was significantly lower than that of the control (no film). Next, we conducted the following in vivo experiments: After the parietal peritoneum was partially removed and covered with each film or without any film, mice were inoculated intraperitoneally with B16 melanoma or Colon26/Nluc cells expressing NanoLuc luciferase gene. At 7 days after the operation, we measured the weight of B16 melanoma tumors or the NanoLuc activity of Colon26/Nluc cells using in vivo imaging at the injured sites. There were no significant differences in the weight of the tumors and the NanoLuc activity among the three groups. We also observed the survival time of mice receiving the same operation and treatments. There was no significant difference in the survival time among the three groups. These results suggest that the gelatin film will likely not accelerate peritoneal dissemination as a convenient scaffold for tumor cell growth when used in surgery for abdominal tumors. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2122-2130, 2018.
|
Volume |
106(6)
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Pages |
2122-2130
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Published |
2018-8-1
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DOI |
10.1002/jbm.b.34017
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PMID |
29024447
|
MeSH |
Animals
Carboxymethylcellulose Sodium / chemistry
Carboxymethylcellulose Sodium / pharmacology
Gelatin* / chemistry
Gelatin* / pharmacology
Hyaluronic Acid / chemistry
Hyaluronic Acid / pharmacology
Male
Melanoma, Experimental* / metabolism
Melanoma, Experimental* / pathology
Melanoma, Experimental* / therapy
Membranes, Artificial*
Mice
Neoplasm Metastasis
Peritoneal Neoplasms* / metabolism
Peritoneal Neoplasms* / pathology
Peritoneal Neoplasms* / therapy
Tissue Adhesions / metabolism
Tissue Adhesions / pathology
Tissue Adhesions / prevention & control*
|
IF |
2.674
|
Times Cited |
0
|
Resource |
Human and Animal Cells |
Colon-26(RCB2657) |