RRC ID 52430
Author Torigoe G, Nagao M, Tanabe N, Manaka S, Kariya T, Kawato T, Sekino J, Kato S, Maeno M, Suzuki N, Shimizu N.
Title PYK2 mediates BzATP-induced extracellular matrix proteins synthesis.
Journal Biochem Biophys Res Commun
Abstract Mechanical stimuli such as fluid shear and cyclic tension force induced extracellular adenosine triphosphate (ATP) release in osteoblasts. In particular, cyclic tension force-induced ATP enhances bone formation through P2X7 activation. Proline-rich tyrosine kinase 2 (PYK2) mediate osteoblasts differentiation is induced by mechanical stimuli. Furthermore, activation of PYK2 also was a response to integrin by mechanical stimuli. Extracellular matrix protein (ECMP)s, which are important factors for bone formation are expressed by osteoblasts. However, the effect of the interaction of 2'(3)-Ο-(4-Benzoylbenzoyl) adenosine-5'-triphosphate (BzATP), which is the agonist of the mechanosensitive receptor P2X7, with PYK2 on ECMP production is poorly understood. Thus, our purpose was to investigate the effects of PYK2 on BzATP-induced ECMP production in osteoblasts. BzATP increased phospho-PYK2 protein expression on days 3 and 7 of culture. Furthermore, the PYK2 inhibitor PF431394 inhibited the stimulatory effect of BzATP on the expression of type I collagen, bone sialoprotein and osteocalcin expression. PF431396 did not inhibit the stimulatory effect of BzATP on osteopontin (OPN) mRNA expression. These results suggest that mechanical stimuli activate P2X7 might induce ECMPs expression through PYK2 except in the case of OPN expression. Altogether, mechanical stimuli-induced ECMPs production might be implicated by extracellular ATP secretion or integrin via PYK2 activation.
Volume 494(3-4)
Pages 663-667
Published 2017-12-16
DOI 10.1016/j.bbrc.2017.10.107
PII S0006-291X(17)32085-5
PMID 29061307
MeSH Adenosine Triphosphate / analogs & derivatives* Adenosine Triphosphate / pharmacology Animals BALB 3T3 Cells Extracellular Matrix / drug effects Extracellular Matrix / physiology* Extracellular Matrix Proteins / biosynthesis* Focal Adhesion Kinase 2 / metabolism* Mechanotransduction, Cellular / genetics Mechanotransduction, Cellular / physiology* Mice Osteoblasts / drug effects Osteoblasts / physiology* Pyrans / agonists Pyrans / metabolism*
IF 2.985
Times Cited 3
Human and Animal Cells MC3T3-E1(RCB1126)