論文 - 詳細
| RRC ID | 52455 |
|---|---|
| 著者 | Matsuo Y, Shinoda D, Nakamaru A, Kamohara K, Sakagami H, Mimaki Y. |
| タイトル | Steroidal Glycosides from Convallaria majalis Whole Plants and Their Cytotoxic Activity. |
| ジャーナル | Int J Mol Sci |
| Abstract |
Phytochemical examination of Convallaria majalis (Liliaceae) whole plants yielded 15 steroidal glycosides (1-15), including nine new compounds (4-6, 10-15) with a lycotetrose unit. The structures of the new compounds were determined using two-dimensional Nuclear magnetic resonance (NMR) analyses and chemical methods. The isolated compounds were evaluated for cytotoxicity against HL-60 human promyelocytic leukemia cells, A549 human lung adenocarcinoma cells, and HSC-4 and HSC-2 human oral squamous cell carcinoma cell lines. Of these, (25S)-spirost-5-en-3β-yl O-β-d-glucopyranosyl-(1→2)-O-[β-d-xylopyranosyl-(1→3)]-O-β-d-glucopyranosyl-(1→4)-β-d-galactopyranoside (1) exhibited cytotoxic activity against HL-60, A549, HSC-4, and HSC-2 cells with IC50 values ranging from 0.96 to 3.15 μM. The corresponding furostanol glycoside of 1, (25S)-26-[(β-d-glucopyranosyl)oxy]-22α-hydroxyfurost-5-en-3β-yl O-β-d-glucopyranosyl-(1→2)-O-[β-d-xylopyranosyl-(1→3)]-O-β-d-glucopyranosyl-(1→4)-β-d-galactopyranoside (8), was cytotoxic to the adherent cell lines of A549, HSC-4, and HSC-2 cells with IC50 values of 2.97, 11.04, and 8.25 μM, respectively. The spirostanol lycotetroside (1) caused necrotic cell death in A549 cells in a dose-dependent manner. Alternatively, the furostanol lycotetroside (8) induced apoptotic cell death in A549 cells in a time-dependent manner, as was evident by morphological observations and flow cytometry analyses. |
| 巻・号 | 18(11) |
| 公開日 | 2017-11-7 |
| DOI | 10.3390/ijms18112358 |
| PII | ijms18112358 |
| PMID | 29112119 |
| PMC | PMC5713327 |
| MeSH | Apoptosis / drug effects Cell Cycle / drug effects Convallaria / chemistry* Flow Cytometry Glycosides / chemistry* Glycosides / pharmacology* HL-60 Cells Humans Magnetic Resonance Spectroscopy |
| IF | 4.556 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HSC-2(RCB1945) HSC-4(RCB1902) |