論文 - 詳細
| RRC ID | 52470 |
|---|---|
| 著者 | Stockler-Pinto MB, Soulage CO, Borges NA, Cardozo LFMF, Dolenga CJ, Nakao LS, Pecoits-Filho R, Fouque D, Mafra D. |
| タイトル | From bench to the hemodialysis clinic: protein-bound uremic toxins modulate NF-κB/Nrf2 expression. |
| ジャーナル | Int Urol Nephrol |
| Abstract |
PURPOSE:Uremic toxins produced by gut microbiota (indoxyl sulfate-IS, p-cresyl sulfate-p-CS, and indole-3-acetic acid-IAA) accumulate in hemodialysis (HD) patients and exhibit potent inflammatory effects. However, the impact of these toxins on nuclear E2-related factor 2 (Nrf2) and nuclear factor-kappa B (NF-κB) expression in HD patients remains poorly defined. The aim of this study was to evaluate the association between uremic toxins and Nrf2/NF-κB expression in vitro (RAW 264.7 macrophage-like cells) and in peripheral blood mononuclear cells from HD patients. METHODS:Uremic toxins, C-reactive protein (CRP), interleukin-6 (IL-6) and malondialdehyde (MDA) levels were measured in fifteen HD patients and nine healthy individuals. RAW 264.7 macrophage-like cells were incubated with IS, as a prototype of protein-bound uremic toxin. Nrf2 and NF-κB expressions were analyzed by RT-qPCR. RESULTS:HD patients presented high levels of inflammatory markers, MDA and uremic toxins. In addition, they presented high NF-κB and low Nrf2 expression. Uremic toxins were positively correlated with NF-κB expression (IS, ρ = 0.58, p < 0.003; p-CS, ρ = 0.71, p < 0.001; IAA, ρ = 0.62, p < 0.001) and negatively with Nrf2 (IS, ρ = - 0.48, p = 0.01; p-CS, ρ = - 0.46, p < 0.02). Uremic toxins also exhibited positive correlations with CRP and MDA levels. Multivariate analysis revealed that p-CS is a determinant factor of NF-κB expression. In RAW 264.7 culture, NF-κB mRNA expression was stimulated by IS, while Nrf2 was downregulated. CONCLUSIONS:Thus, uremic toxins may stimulate NF-κB mRNA and decrease Nrf2 expression in HD patients and, consequently, trigger inflammation and oxidative stress. |
| 巻・号 | 50(2) |
| ページ | 347-354 |
| 公開日 | 2018-2-1 |
| DOI | 10.1007/s11255-017-1748-y |
| PII | 10.1007/s11255-017-1748-y |
| PMID | 29151180 |
| MeSH | Aged Animals Cell Culture Techniques Female Humans Inflammation / blood Inflammation / metabolism Interleukin-6 / blood Male Malondialdehyde / blood Mice Middle Aged NF-E2-Related Factor 2 / metabolism* NF-kappa B / metabolism* Oxidative Stress / physiology Renal Dialysis / adverse effects Renal Dialysis / methods Renal Insufficiency, Chronic* / blood Renal Insufficiency, Chronic* / diagnosis Renal Insufficiency, Chronic* / therapy Signal Transduction Uremia* / etiology Uremia* / metabolism |
| IF | 1.692 |
| 引用数 | 12 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |