| RRC ID |
52472
|
| 著者 |
Hikita K, Hattori N, Takeda A, Yamakage Y, Shibata R, Yamada S, Kato K, Murata T, Tanaka H, Kaneda N.
|
| タイトル |
Potent apoptosis-inducing activity of erypoegin K, an isoflavone isolated from Erythrina poeppigiana, against human leukemia HL-60 cells.
|
| ジャーナル |
J Nat Med
|
| Abstract |
Erypoegin K is an isoflavone isolated from the stem bark of Erythrina poeppigiana. It contains a furan group at the A-ring of the core isoflavone structure and can inhibit the activity of glyoxalase I, an enzyme that catalyzes the detoxification of methylglyoxal (MG), a by-product of glycolysis. In the present study, we found that erypoegin K has a potent cytotoxic effect on human leukemia HL-60 cells. Its cytotoxic effect was much stronger than that of a known glyoxalase I inhibitor S-p-bromobenzylglutathione cyclopentyl diester. Conversely, erypoegin K demonstrated weak cytotoxicity toward normal human peripheral lymphocytes. The treatment of HL-60 cells with erypoegin K significantly induced caspase-3 activity, whereas the pretreatment of the cells with caspase-3 inhibitor suppressed erypoegin K-induced cell death. Furthermore, nuclear condensation and apoptotic genome DNA fragmentation were observed in erypoegin K-treated HL-60 cells. These results indicated that the observed cell death was mediated by apoptosis. In addition, the toxic compound MG was highly accumulated in the culture medium of erypoegin K-treated HL-60 cells, suggesting that cell apoptosis was triggered by extracellular MG. The present study showed that erypoegin K has a potent apoptosis-inducing effect on cancerous cell lines, such as HL-60.
|
| 巻・号 |
72(1)
|
| ページ |
260-266
|
| 公開日 |
2018-1-1
|
| DOI |
10.1007/s11418-017-1147-9
|
| PII |
10.1007/s11418-017-1147-9
|
| PMID |
29151157
|
| MeSH |
Apoptosis
Benzofurans / chemistry*
Erythrina / chemistry*
HL-60 Cells / chemistry*
Humans
Isoflavones / chemistry*
Leukemia / drug therapy*
Leukemia / pathology
|
| IF |
2.055
|
| 引用数 |
2
|
| リソース情報 |
| ヒト・動物細胞 |
HL-60(RCB3683) |
