RRC ID 52473
Author Villareal MO, Kume S, Neffati M, Isoda H.
Title Upregulation of Mitf by Phenolic Compounds-Rich Cymbopogon schoenanthus Treatment Promotes Melanogenesis in B16 Melanoma Cells and Human Epidermal Melanocytes.
Journal Biomed Res Int
Abstract Melanin provides inherent protection against skin cancer by absorbing broad-spectrum radiant energy of UV radiation. Cutaneous malignant melanoma incidence has recently been observed to increase and the frequency is closely associated with the skin color, highlighting the importance of skin pigmentation. Here, we showed how melanin biosynthesis is enhanced by treatment with phenolic compounds-rich Cymbopogon schoenanthus (CYM) in B16 murine melanoma cells and human epidermal melanocytes (HEM). CYM increased the melanin content of the cells by upregulating the expression of tyrosinase (TYR), tyrosinase-related protein 1 (TRP1), and dopachrome tautomerase (DCT) at the protein and mRNA levels, comparable to the effect of α-melanocyte-stimulating hormone (MSH), in both B16 cells and HEM. Moreover, global gene expression analysis showed that at least 44 pigmentation-associated genes were modulated, including the microphthalmia-associated transcription factor (Mitf) and its transcriptional regulators (Sox10, Pax3, and Lef1). Upregulation of copper transport-associated gene Atp7b indicates that CYM also promotes tyrosinase activity. CYM upregulated Mitf and possibly activates tyrosinase enzyme, providing evidence for its possible use to promote melanogenesis and as a therapeutic agent against hypopigmentation disorders.
Volume 2017
Pages 8303671
Published 2017-11-21
DOI 10.1155/2017/8303671
PMID 29359158
PMC PMC5735589
MeSH Animals Cell Line Cell Proliferation / drug effects Cymbopogon / chemistry* Epidermal Cells Humans Melanocytes / drug effects* Melanocytes / metabolism Mice Microphthalmia-Associated Transcription Factor / genetics* Microphthalmia-Associated Transcription Factor / metabolism Phenols / chemistry Phenols / pharmacology* Plant Extracts / chemistry Plant Extracts / pharmacology Transcriptome / drug effects Up-Regulation / drug effects*
IF 2.197
Human and Animal Cells B16 F10(RCB2630)