論文 - 詳細
| RRC ID | 52482 |
|---|---|
| 著者 | Nishimura K, Goto K, Nakagawa H. |
| タイトル | Effect of erythropoietin production induced by hypoxia on autophagy in HepG2 cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
The induction of hypoxia inducible factor (HIF) and autophagy are important cellular responses to hypoxia. The production of erythropoietin (EPO) regulated by HIF is increased by hypoxia and participates in cell protection in various organs and tissues. Signal Transducers and Activator of Transcription 3 (STAT3) is a regulatory factor that is common to autophagy induction and EPO-EPO receptor signaling. In this study, we analyzed the promotion of EPO production and autophagy, and the participation of STAT3, in HepG2 cells under hypoxia. Treatment with EPO siRNA (si-EPO) significantly increased autophagy induction by hypoxia, while treatment with recombinant EPO inhibited the effect of si-EPO. NSC74859, an inhibitor of the phosphorylation of STAT3, increased autophagy induction to the same extent as si-EPO treatment. Even when 3-Methyladenine, an inhibitor of autophagy, was added, the increase of EPO mRNA expression due to hypoxia was not affected. Hypoxia-induced EPO restrained autophagy induction through the EPO receptor and phosphorylation of STAT3. Because cell viability with treatment of si-EPO under hypoxia did not increase over the control, our results suggested that EPO produced by hypoxia prevented excess autophagy induction. |
| 巻・号 | 495(1) |
| ページ | 1317-1321 |
| 公開日 | 2018-1-1 |
| DOI | 10.1016/j.bbrc.2017.11.167 |
| PII | S0006-291X(17)32345-8 |
| PMID | 29191652 |
| MeSH | Autophagy* Cell Hypoxia* Erythropoietin / biosynthesis* Hep G2 Cells Humans STAT3 Transcription Factor / metabolism* Signal Transduction* |
| IF | 2.985 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB1648) |