論文 - 詳細
| RRC ID | 52494 |
|---|---|
| 著者 | Tabe S, Hikiji H, Ariyoshi W, Hashidate-Yoshida T, Shindou H, Shimizu T, Okinaga T, Seta Y, Tominaga K, Nishihara T. |
| タイトル | Lysophosphatidylcholine acyltransferase 4 is involved in chondrogenic differentiation of ATDC5 cells. |
| ジャーナル | Sci Rep |
| Abstract |
Glycerophospholipids have important structural and functional roles in cells and are the main components of cellular membranes. Glycerophospholipids are formed via the de novo pathway (Kennedy pathway) and are subsequently matured in the remodeling pathway (Lands' cycle). Lands' cycle consists of two steps: deacylation of phospholipids by phospholipases A2 and reacylation of lysophospholipids by lysophospholipid acyltransferases (LPLATs). LPLATs play key roles in the maturation and maintenance of the fatty acid composition of biomembranes, and cell differentiation. We examined whether LPLATs are involved in chondrogenic differentiation of ATDC5 cells, which can differentiate into chondrocytes. Lysophosphatidylcholine acyltransferase 4 (LPCAT4) mRNA expression and LPCAT enzymatic activity towards 18:1-, 18:2-, 20:4-, and 22:6-CoA increased in the late stage of chondrogenic differentiation, when mineralization occurred. LPCAT4 knockdown decreased mRNA and protein levels of chondrogenic markers as well as Alcian blue staining intensity and alkaline phosphatase activity in ATDC5 cells. These results suggest that LPCAT4 plays important roles during the transition of chondrocytes into hypertrophic chondrocytes and/or a mineralized phenotype. |
| 巻・号 | 7(1) |
| ページ | 16701 |
| 公開日 | 2017-12-1 |
| DOI | 10.1038/s41598-017-16902-4 |
| PII | 10.1038/s41598-017-16902-4 |
| PMID | 29196633 |
| PMC | PMC5711957 |
| MeSH | 1-Acylglycerol-3-Phosphate O-Acyltransferase / antagonists & inhibitors 1-Acylglycerol-3-Phosphate O-Acyltransferase / genetics 1-Acylglycerol-3-Phosphate O-Acyltransferase / metabolism* Animals Bone Morphogenetic Protein 2 / genetics Bone Morphogenetic Protein 2 / metabolism Cell Differentiation Cell Line Chondrogenesis* Core Binding Factor Alpha 1 Subunit / genetics Core Binding Factor Alpha 1 Subunit / metabolism Mice RNA Interference RNA, Small Interfering / metabolism |
| IF | 3.998 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) 10T1/2(RCB0247) |