論文 - 詳細
| RRC ID | 52499 |
|---|---|
| 著者 | Suzuki T, Maeda S, Furuhata E, Shimizu Y, Nishimura H, Kishima M, Suzuki H. |
| タイトル | A screening system to identify transcription factors that induce binding site-directed DNA demethylation. |
| ジャーナル | Epigenetics Chromatin |
| Abstract |
BACKGROUND:DNA methylation is a fundamental epigenetic modification that is involved in many biological systems such as differentiation and disease. We and others recently showed that some transcription factors (TFs) are involved in the site-specific determination of DNA demethylation in a binding site-directed manner, although the reports of such TFs are limited. RESULTS:Here, we develop a screening system to identify TFs that induce binding site-directed DNA methylation changes. The system involves the ectopic expression of target TFs in model cells followed by DNA methylome analysis and overrepresentation analysis of the corresponding TF binding motif at differentially methylated regions. It successfully identified binding site-directed demethylation of SPI1, which is known to promote DNA demethylation in a binding site-directed manner. We extended our screening system to 15 master TFs involved in cellular differentiation and identified eight novel binding site-directed DNA demethylation-inducing TFs (RUNX3, GATA2, CEBPB, MAFB, NR4A2, MYOD1, CEBPA, and TBX5). Gene ontology and tissue enrichment analysis revealed that these TFs demethylate genomic regions associated with corresponding biological roles. We also describe the characteristics of binding site-directed DNA demethylation induced by these TFs, including the targeting of highly methylated CpGs, local DNA demethylation, and the overlap of demethylated regions between TFs of the same family. CONCLUSIONS:Our results show the usefulness of the developed screening system for the identification of TFs that induce DNA demethylation in a site-directed manner. |
| 巻・号 | 10(1) |
| ページ | 60 |
| 公開日 | 2017-12-8 |
| DOI | 10.1186/s13072-017-0169-6 |
| PII | 10.1186/s13072-017-0169-6 |
| PMID | 29221486 |
| PMC | PMC5723091 |
| MeSH | Binding Sites DNA Methylation Demethylation* Transcription Factors / metabolism* |
| IF | 4.237 |
| 引用数 | 14 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |