論文 - 詳細
| RRC ID | 525 |
|---|---|
| 著者 | Fujimoto H, Hirukawa Y, Tani H, Matsuura Y, Hashido K, Tsuchida K, Takada N, Kobayashi M, Maekawa H. |
| タイトル | Integration of the 5' end of the retrotransposon, R2Bm, can be complemented by homologous recombination. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
R2Bm is a non-long-terminal-repeat (non-LTR) retrotransposon that was identified at a specific target site in the 28S rRNA genes of the silkworm, Bombyx mori. Although in vitro analysis has revealed that the 3' end of R2Bm is integrated into the target site by means of target-primed reverse transcription (TPRT), the mechanism of the 5' end integration is not well understood. We established a novel in vivo system to assay the insertion mechanism of R2Bm using a cultured cell line, C65, and a baculovirus, AcNPV, as host and vector, respectively. The 3' end of R2Bm integrated at the target site in the rRNA genes of C65 cells when an AcNPV containing both the full-length 3' UTR and the entire open reading frame (ORF) of R2Bm was introduced while the 5' end integration was incorrect. The 5' end of R2Bm was integrated, however, when the 28S gene sequence upstream of the R2Bm target site was added to the R2Bm sequence. Thus, in our assay, homologous sequences were likely essential for the successful integration of the entire R2Bm into the host cell genome. We also demonstrated that the failure to integrate caused by a frame-shifted ORF was rescued by co-infection with a helper virus that contained only the R2Bm ORF. This indicates that R2 retrotransposition can be complemented in trans. These findings suggest that the host's mechanism for DNA repair may be necessary for the integration of the 5' end of R2Bm and that R2Bm protein may only have the ability to integrate the 3' end of the element by TPRT. |
| 巻・号 | 32(4) |
| ページ | 1555-65 |
| 公開日 | 2004-1-1 |
| DOI | 10.1093/nar/gkh304 |
| PII | 32/4/1555 |
| PMID | 14999096 |
| PMC | PMC390292 |
| MeSH | Animals Cell Line DNA Repair Frameshift Mutation Genome Helper Viruses / genetics Molecular Sequence Data Mutagenesis, Insertional* Recombination, Genetic* Retroelements* Transcription, Genetic |
| IF | 11.502 |
| 引用数 | 15 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 19 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| カイコ | w30 EST database |