| RRC ID |
52544
|
| 著者 |
Iwai S, Kishimoto S, Amano Y, Nishiguchi A, Matsusaki M, Takeshita A, Akashi M.
|
| タイトル |
Three-dimensional cultured tissue constructs that imitate human living tissue organization for analysis of tumor cell invasion.
|
| ジャーナル |
J Biomed Mater Res A
|
| Abstract |
Preventing cancer metastasis requires a thorough understanding of cancer cell invasion. These phenomena occur in human 3-D living tissues. To this end, we developed a human cell-based three-dimensional (3-D) cultured tissue constructs that imitate in vivo human tissue organization. We investigated whether our 3-D cell culture system can be used to analyze the invasion of human oral squamous cell carcinoma (OSCC) cells. The 3-D tissue structure consisted of five layers of normal human dermal fibroblasts along with human dermal lymphatic endothelial cell tubes and was generated by the cell accumulation technique and layer-by-layer assembly using fibronectin and gelatin. OSCC cells with different lymph metastatic capacity were inoculated on the 3-D tissues and their invasion through the 3-D tissue structure was observed. Conventional methods of analyzing cell migration and invasion, that is, 2-D culture-based transwell and Matrigel assays were also used for comparison. The results using the 3-D cultured tissue constructs were comparable to those obtained using conventional assays; moreover, use of the 3-D system enabled visualization of differential invasion capacities of cancer cells. These results indicate that our 3-D cultured tissue constructs can be a useful tool for analysis of cancer cell invasion in a setting that reflects the in vivo tissue organization. © 2018 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 292-300, 2019.
|
| 巻・号 |
107(2)
|
| ページ |
292-300
|
| 公開日 |
2019-2-1
|
| DOI |
10.1002/jbm.a.36319
|
| PMID |
29280265
|
| PMC |
PMC6587574
|
| MeSH |
Biocompatible Materials / chemistry
Carcinoma, Squamous Cell / pathology*
Cell Culture Techniques / methods*
Cell Line, Tumor
Cell Movement
Fibronectins / chemistry
Gelatin / chemistry
Humans
Mouth Neoplasms / pathology*
Neoplasm Invasiveness / pathology*
Tissue Scaffolds / chemistry
|
| IF |
3.525
|
| 引用数 |
2
|
| リソース情報 |
| ヒト・動物細胞 |
HSC-3(RCB1975) |
