論文 - 詳細
| RRC ID | 52544 |
|---|---|
| 著者 | Iwai S, Kishimoto S, Amano Y, Nishiguchi A, Matsusaki M, Takeshita A, Akashi M. |
| タイトル | Three-dimensional cultured tissue constructs that imitate human living tissue organization for analysis of tumor cell invasion. |
| ジャーナル | J Biomed Mater Res A |
| Abstract |
Preventing cancer metastasis requires a thorough understanding of cancer cell invasion. These phenomena occur in human 3-D living tissues. To this end, we developed a human cell-based three-dimensional (3-D) cultured tissue constructs that imitate in vivo human tissue organization. We investigated whether our 3-D cell culture system can be used to analyze the invasion of human oral squamous cell carcinoma (OSCC) cells. The 3-D tissue structure consisted of five layers of normal human dermal fibroblasts along with human dermal lymphatic endothelial cell tubes and was generated by the cell accumulation technique and layer-by-layer assembly using fibronectin and gelatin. OSCC cells with different lymph metastatic capacity were inoculated on the 3-D tissues and their invasion through the 3-D tissue structure was observed. Conventional methods of analyzing cell migration and invasion, that is, 2-D culture-based transwell and Matrigel assays were also used for comparison. The results using the 3-D cultured tissue constructs were comparable to those obtained using conventional assays; moreover, use of the 3-D system enabled visualization of differential invasion capacities of cancer cells. These results indicate that our 3-D cultured tissue constructs can be a useful tool for analysis of cancer cell invasion in a setting that reflects the in vivo tissue organization. © 2018 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 292-300, 2019. |
| 巻・号 | 107(2) |
| ページ | 292-300 |
| 公開日 | 2019-2-1 |
| DOI | 10.1002/jbm.a.36319 |
| PMID | 29280265 |
| PMC | PMC6587574 |
| MeSH | Biocompatible Materials / chemistry Carcinoma, Squamous Cell / pathology* Cell Culture Techniques / methods* Cell Line, Tumor Cell Movement Fibronectins / chemistry Gelatin / chemistry Humans Mouth Neoplasms / pathology* Neoplasm Invasiveness / pathology* Tissue Scaffolds / chemistry |
| IF | 3.525 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HSC-3(RCB1975) |