| RRC ID |
52552
|
| 著者 |
Kunimatsu R, Gunji H, Tsuka Y, Yoshimi Y, Awada T, Sumi K, Nakajima K, Kimura A, Hiraki T, Abe T, Naoto H, Yanoshita M, Tanimoto K.
|
| タイトル |
Effects of high-frequency near-infrared diode laser irradiation on the proliferation and migration of mouse calvarial osteoblasts.
|
| ジャーナル |
Lasers Med Sci
|
| Abstract |
Laser irradiation activates a range of cellular processes and can promote tissue repair. Here, we examined the effects of high-frequency near-infrared (NIR) diode laser irradiation on the proliferation and migration of mouse calvarial osteoblastic cells (MC3T3-E1). MC3T3-E1 cells were cultured and exposed to high-frequency (30 kHz) 910-nm diode laser irradiation at a dose of 0, 1.42, 2.85, 5.7, or 17.1 J/cm2. Cell proliferation was evaluated with BrdU and ATP concentration assays. Cell migration was analyzed by quantitative assessment of wound healing using the Incucyt® ZOOM system. In addition, phosphorylation of mitogen-activated protein kinase (MAPK) family members including p38 mitogen-activated protein kinase (p38), stress-activated protein kinase/Jun-amino-terminal kinase (SAPK/JNK), and extracellular signal-regulated protein kinase (ERK)1/2) after laser irradiation was examined with western blotting. Compared to the control, cell proliferation was significantly increased by laser irradiation at a dose of 2.85, 5.7, or 17.1 J/cm2. Laser irradiation at a dose of 2.85 J/cm2 induced MC3T3-E1 cells to migrate more rapidly than non-irradiated control cells. Irradiation with the high-frequency 910-nm diode laser at a dose of 2.85 J/cm2 induced phosphorylation of MAPK/ERK1/2 15 and 30 min later. However, phosphorylation of p38 MAPK and SAPK/JNK was not changed by NIR diode laser irradiation at a dose of 2.85 J/cm2. Irradiation with a high-frequency NIR diode laser increased cell division and migration of MT3T3-E1 cells, possibly via MAPK/ERK signaling. These observations may be important for enhancing proliferation and migration of osteoblasts to improve regeneration of bone tissues.
|
| 巻・号 |
33(5)
|
| ページ |
959-966
|
| 公開日 |
2018-7-1
|
| DOI |
10.1007/s10103-017-2426-0
|
| PII |
10.1007/s10103-017-2426-0
|
| PMID |
29302842
|
| MeSH |
Adenosine Triphosphate / biosynthesis
Animals
Cell Line
Cell Movement / radiation effects*
Cell Proliferation / radiation effects
DNA / biosynthesis
Infrared Rays*
Lasers, Semiconductor*
Mice
Osteoblasts / cytology*
Osteoblasts / radiation effects*
Signal Transduction / radiation effects
Skull / cytology*
|
| IF |
2.342
|
| 引用数 |
4
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
