論文 - 詳細
| RRC ID | 52560 |
|---|---|
| 著者 | Mita P, Wudzinska A, Sun X, Andrade J, Nayak S, Kahler DJ, Badri S, LaCava J, Ueberheide B, Yun CY, Fenyö D, Boeke JD. |
| タイトル | LINE-1 protein localization and functional dynamics during the cell cycle. |
| ジャーナル | Elife |
| Abstract |
LINE-1/L1 retrotransposon sequences comprise 17% of the human genome. Among the many classes of mobile genetic elements, L1 is the only autonomous retrotransposon that still drives human genomic plasticity today. Through its co-evolution with the human genome, L1 has intertwined itself with host cell biology. However, a clear understanding of L1's lifecycle and the processes involved in restricting its insertion and intragenomic spread remains elusive. Here we identify modes of L1 proteins' entrance into the nucleus, a necessary step for L1 proliferation. Using functional, biochemical, and imaging approaches, we also show a clear cell cycle bias for L1 retrotransposition that peaks during the S phase. Our observations provide a basis for novel interpretations about the nature of nuclear and cytoplasmic L1 ribonucleoproteins (RNPs) and the potential role of DNA replication in L1 retrotransposition. |
| 巻・号 | 7 |
| 公開日 | 2018-1-8 |
| DOI | 10.7554/eLife.30058 |
| PII | 30058 |
| PMID | 29309036 |
| PMC | PMC5821460 |
| MeSH | Cell Cycle* Cell Nucleus / metabolism* Humans Long Interspersed Nucleotide Elements Protein Transport Ribonucleoproteins / metabolism* |
| IF | 7.08 |
| 引用数 | 29 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 42 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa/Fucci(RCB2812) |