論文 - 詳細
| RRC ID | 52564 |
|---|---|
| 著者 | Egami Y, Araki N. |
| タイトル | Transient recruitment of M-Ras GTPase to phagocytic cups in RAW264 macrophages during FcγR-mediated phagocytosis. |
| ジャーナル | Microscopy (Oxf) |
| Abstract |
M-Ras, a member of the Ras superfamily, is known to be involved in diverse cellular processes. However, its involvement in FcγR-mediated phagocytosis remains unknown. We examined the spatiotemporal localization of M-Ras during the engulfment of IgG-opsonized erythrocytes (IgG-Es) in RAW264 macrophages. By the live-cell imaging of fluorescent protein-fused M-Ras, we found that M-Ras was localized to the membrane of phagocytic cups during the early stage of phagosome formation. Notably, ratiometric image analysis revealed that M-Ras was concentrated in the membrane of forming phagosomes. Moreover, our analysis of M-Ras mutant expression showed that phagosome formation was significantly inhibited in cells expressing GDP-locked mutant M-Ras-S27N. In contrast, the expression of wild-type M-Ras or GTP-locked mutant M-Ras-G22V facilitated the uptake of IgG-Es. These data suggest that M-Ras is a novel component of the FcγR-mediated phagocytic pathway and may regulate phagosome formation in macrophages. |
| 巻・号 | 67(2) |
| ページ | 68-74 |
| 公開日 | 2018-4-1 |
| DOI | 10.1093/jmicro/dfx131 |
| PII | 4797532 |
| PMID | 29340604 |
| MeSH | Animals Cell Line Cell Membrane / immunology Erythrocytes / immunology* Immunoglobulin G / immunology Macrophages / immunology* Mice Monomeric GTP-Binding Proteins / metabolism* Phagocytosis / immunology* Phagosomes / immunology Protein Binding / immunology RAW 264.7 Cells Receptors, IgG / immunology* ras Proteins |
| IF | 1.394 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |