RRC ID 52579
著者 Nakamura A, Matsunaga W, Gotoh A.
タイトル Autophagy Induced by Naftopidil Inhibits Apoptosis of Human Gastric Cancer Cells.
ジャーナル Anticancer Res
Abstract AIM:Naftopidil is used to treat benign prostate hyperplasia. Moreover, previous studies have shown that naftopidil reduced viability of many types of cancer cells. Therefore, we investigated the antitumor mechanism of naftopidil in this study.
MATERIALS AND METHODS:We used the HGC27 human gastric cancer cell line. It was treated with naftopidil, pan-caspase inhibitor, and chloroquine diphosphate (CQ). Cell viability and cell death were investigated by the assay and annexin V/ propidium iodide assay. Phosphorylation of protein kinase B (AKT) (Ser473) was measured by western blotting. Alteration of light chain 3B (LC3B) was investigated by western blotting and immunofluorescence.
RESULTS:Naftopidil reduced phospho-AKT (Ser473) and altered LC3B. Combination of naftopidil and CQ reduced cell viability and phospho-AKT (Ser 473).
CONCLUSION:Naftopidil induces apoptosis and autophagy of HGC27 cells, however, autophagy is considered to inhibit apoptosis. We concluded naftopidil and CQ have a synergistic antitumor effect.
巻・号 38(2)
ページ 803-809
公開日 2018-2-1
DOI 10.21873/anticanres.12287
PII 38/2/803
PMID 29374705
MeSH Antineoplastic Combined Chemotherapy Protocols / pharmacology* Apoptosis / drug effects* Autophagy / drug effects* Cell Line, Tumor Chloroquine / administration & dosage Chloroquine / analogs & derivatives Chloroquine / pharmacology Drug Synergism Humans Microtubule-Associated Proteins / metabolism Naphthalenes / administration & dosage Naphthalenes / pharmacology* Phosphorylation Piperazines / administration & dosage Piperazines / pharmacology* Proto-Oncogene Proteins c-akt / metabolism Stomach Neoplasms / drug therapy* Stomach Neoplasms / metabolism Stomach Neoplasms / pathology
IF 1.994
引用数 1
リソース情報
ヒト・動物細胞 HGC-27(RCB0500)