論文 - 詳細
RRC ID | 52606 |
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著者 | Li F, Sun J, Huang S, Su G, Pi G. |
タイトル | LncRNA GAS5 Overexpression Reverses LPS-Induced Inflammatory Injury and Apoptosis Through Up-Regulating KLF2 Expression in ATDC5 Chondrocytes. |
ジャーナル | Cell Physiol Biochem |
Abstract |
BACKGROUND/AIMS:Osteoarthritis (OA) is the most frequently occurring joint disease and characterized by degeneration of cartilage. As the unique cell type in cartilage, chondrocytes play a crucial role during OA. Our study explored the influence of long non-coding RNA (lncRNA) growth arrest-specific transcript 5 (GAS5) on lipopolysaccharides (LPS)-induced injury in ATDC5 cells. METHODS:Cell viability, apoptosis and expression of inflammatory cytokines were all assessed to evaluate LPS-induce inflammatory injury. Expression of GAS5 in LPS-induced cells was evaluated by qRT-PCR. After cell transfection, effect of abnormally expressed GAS5 on LPS-induced inflammatory injury was determined. Then, the possible target of GAS5 was screened by bioinformatics and verified by qRT-PCR and luciferase activity assay. Together, whether aberrant expression of target gene affected the modulation of GAS5 in LPS-induced inflammatory injury was also assessed. Finally, the influences of aberrant expressed Kruppel-like factor 2 (KLF2) on nuclear factor κB (NF-κB) and Notch pathways were detected by Western blot analysis. RESULTS:LPS reduced cell viability and promoted cell apoptosis and secretion of inflammatory cytokines, along with down-regulation of GAS5. LPS-induced injury was alleviated by GAS5 overexpression while was exacerbated by GAS5 silence. KLF2 was predicted and verified as a target of GAS5, and GAS5 functioned through regulating expression of KLF2. Besides, aberrant expression of KLF2 regulated expressions of key kinases involved in the NF-κB and Notch pathways. CONCLUSION:GAS5 might ameliorate LPS-induced inflammatory injury in ATDC5 chondrocytes by inhibiting the NF-κB and Notch signaling pathways. |
巻・号 | 45(3) |
ページ | 1241-1251 |
公開日 | 2018-2-9 |
DOI | 10.1159/000487455 |
PII | 000487455 |
PMID | 29448248 |
MeSH | Animals Apoptosis / drug effects* Cell Line Chondrocytes / cytology Chondrocytes / drug effects Chondrocytes / metabolism Down-Regulation / drug effects Humans Interleukin-1beta / analysis Interleukin-6 / analysis Interleukin-8 / analysis Kruppel-Like Transcription Factors / genetics* Kruppel-Like Transcription Factors / metabolism Lipopolysaccharides / toxicity* Mice NF-kappa B / metabolism RNA, Long Noncoding / genetics RNA, Long Noncoding / metabolism* Receptors, Notch / metabolism Tumor Necrosis Factor-alpha / analysis Up-Regulation / drug effects* |
IF | 5.5 |
引用数 | 27 |
リソース情報 | |
ヒト・動物細胞 | ATDC5(RCB0565) |