論文 - 詳細
| RRC ID | 52607 |
|---|---|
| 著者 | Okuda T, Fukui A. |
| タイトル | Generation of anti-oligosaccharide antibodies that recognize mammalian glycoproteins by immunization with a novel artificial glycosphingolipid. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Here we report a new method for the efficient generation of antibodies that recognize the fine structures of oligosaccharides on glycoproteins. We found a newly designed artificial glycosphingolipid carrying a very long chain fatty acid to be a strong immunogen in mice, with the serum of immunized mice containing antibodies recognizing the oligosaccharide structure of the immunogen. First, we found that conjugation of a simple ceramide analogue to target oligosaccharides could enhance the immunogenicity of these oligosaccharides in these immunized mice. This effect was confirmed in mice immunized with the artificial glycosphingolipids carrying 6'-Sialyl-LacNAc, 3'-Sialyl-LacNAc and LacNAc. Next, we tried to improve the immunogenic enhancing effect of the ceramide analogue. In a model experiment using 6'-Sialyl-LacNAc oligosaccharide, we manipulated the alkyl chains to several lengths, and found that a longer alkyl chain length of the fatty acid correlated with high immunogenicity. Among these we examined, artificial glycosphingolipids conjugated with a ceramide analogue carrying a very long chain fatty acid (lignoceric acid) showed the strongest immunogenicity. By using the artificial glycosphingolipid containing 6'-Sialy-LacNAc and lignoceric acid, we succeeded in the generation of a new anti-6'-Sialyl-LacNAc antibody that recognizes 6'-Sialyl-LacNAc carrying glycoproteins but does not bind to 6'-Sialyllactose, asialo-glycoporoteins and glycoproteins carrying 3'-Sialyl-LacNAc. These results indicate that the established technology is valuable for the targeted generation of monoclonal antibodies against glycoproteins containing specific oligosaccharide structures. |
| 巻・号 | 497(4) |
| ページ | 983-989 |
| 公開日 | 2018-3-18 |
| DOI | 10.1016/j.bbrc.2018.02.113 |
| PII | S0006-291X(18)30342-5 |
| PMID | 29458022 |
| MeSH | Animals Antibodies, Monoclonal / immunology* Glycoproteins / chemistry Glycoproteins / immunology Glycosphingolipids / chemical synthesis Glycosphingolipids / chemistry Glycosphingolipids / immunology Immunization Methods Mice Oligosaccharides / immunology* Sensitivity and Specificity |
| IF | 2.985 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Sp2/0-Ag14(RCB0209) |