Reference - Detail
| RRC ID | 52641 |
|---|---|
| Author | He Q, Okajima T, Onoe H, Subagyo A, Sueoka K, Kuribayashi-Shigetomi K. |
| Title | Origami-based self-folding of co-cultured NIH/3T3 and HepG2 cells into 3D microstructures. |
| Journal | Sci Rep |
| Abstract |
This paper describes an origami-inspired self-folding method to form three-dimensional (3D) microstructures of co-cultured cells. After a confluent monolayer of fibroblasts (NIH/3T3 cells) with loaded hepatocytes (HepG2 cells) was cultured onto two-dimensional (2D) microplates, degradation of the alginate sacrificial layer in the system by addition of alginate lyase triggered NIH/3T3 cells to self-fold the microplates around HepG2 cells, and then 3D cell co-culture microstructures were spontaneously formed. Using this method, we can create a large number of 3D cell co-culture microstructures swiftly with ease in the same time. We find that HepG2 cells confined in the 3D cell co-culture microstructures have an ability to enhance the secreted albumin compared to 2D system in a long culture period. The result indicates that the origami-based cell self-folding technique presented here is useful in regenerative medicine and the preclinical stage of drug development. |
| Volume | 8(1) |
| Pages | 4556 |
| Published | 2018-3-14 |
| DOI | 10.1038/s41598-018-22598-x |
| PII | 10.1038/s41598-018-22598-x |
| PMID | 29540810 |
| PMC | PMC5852161 |
| MeSH | Animals Cell Shape Cell Survival Coculture Techniques / instrumentation* Coculture Techniques / methods Hep G2 Cells Humans Mice NIH 3T3 Cells Regenerative Medicine Serum Albumin, Human / metabolism* |
| IF | 3.998 |
| Times Cited | 11 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | NIH/3T3(RCB2767) Hep G2(RCB1886) |