| RRC ID |
52641
|
| Author |
He Q, Okajima T, Onoe H, Subagyo A, Sueoka K, Kuribayashi-Shigetomi K.
|
| Title |
Origami-based self-folding of co-cultured NIH/3T3 and HepG2 cells into 3D microstructures.
|
| Journal |
Sci Rep
|
| Abstract |
This paper describes an origami-inspired self-folding method to form three-dimensional (3D) microstructures of co-cultured cells. After a confluent monolayer of fibroblasts (NIH/3T3 cells) with loaded hepatocytes (HepG2 cells) was cultured onto two-dimensional (2D) microplates, degradation of the alginate sacrificial layer in the system by addition of alginate lyase triggered NIH/3T3 cells to self-fold the microplates around HepG2 cells, and then 3D cell co-culture microstructures were spontaneously formed. Using this method, we can create a large number of 3D cell co-culture microstructures swiftly with ease in the same time. We find that HepG2 cells confined in the 3D cell co-culture microstructures have an ability to enhance the secreted albumin compared to 2D system in a long culture period. The result indicates that the origami-based cell self-folding technique presented here is useful in regenerative medicine and the preclinical stage of drug development.
|
| Volume |
8(1)
|
| Pages |
4556
|
| Published |
2018-3-14
|
| DOI |
10.1038/s41598-018-22598-x
|
| PII |
10.1038/s41598-018-22598-x
|
| PMID |
29540810
|
| PMC |
PMC5852161
|
| MeSH |
Animals
Cell Shape
Cell Survival
Coculture Techniques / instrumentation*
Coculture Techniques / methods
Hep G2 Cells
Humans
Mice
NIH 3T3 Cells
Regenerative Medicine
Serum Albumin, Human / metabolism*
|
| IF |
3.998
|
| Times Cited |
11
|
| Resource |
| Human and Animal Cells |
NIH/3T3(RCB2767)
Hep G2(RCB1886) |
