Reference - Detail
| RRC ID | 52663 |
|---|---|
| Author | Tsunoyama TA, Watanabe Y, Goto J, Naito K, Kasai RS, Suzuki KGN, Fujiwara TK, Kusumi A. |
| Title | Super-long single-molecule tracking reveals dynamic-anchorage-induced integrin function. |
| Journal | Nat Chem Biol |
| Abstract |
Single-fluorescent-molecule imaging tracking (SMT) is becoming an important tool to study living cells. However, photobleaching and photoblinking (hereafter referred to as photobleaching/photoblinking) of the probe molecules strongly hamper SMT studies of living cells, making it difficult to observe in vivo molecular events and to evaluate their lifetimes (e.g., off rates). The methods used to suppress photobleaching/photoblinking in vitro are difficult to apply to living cells because of their toxicities. Here using 13 organic fluorophores we found that, by combining low concentrations of dissolved oxygen with a reducing-plus-oxidizing system, photobleaching/photoblinking could be strongly suppressed with only minor effects on cells, which enabled SMT for as long as 12,000 frames (~7 min at video rate, as compared to the general 10-s-order durations) with ~22-nm single-molecule localization precisions. SMT of integrins revealed that they underwent temporary (<80-s) immobilizations within the focal adhesion region, which were responsible for the mechanical linkage of the actin cytoskeleton to the extracellular matrix. |
| Volume | 14(5) |
| Pages | 497-506 |
| Published | 2018-5-1 |
| DOI | 10.1038/s41589-018-0032-5 |
| PII | 10.1038/s41589-018-0032-5 |
| PMID | 29610485 |
| MeSH | Actin Cytoskeleton / metabolism Animals CHO Cells Cell Adhesion Cricetulus Extracellular Matrix / metabolism Fluorescent Dyes / chemistry* HeLa Cells Humans Integrin beta1 / metabolism Integrin beta3 / metabolism Integrins / metabolism* Mice Microscopy, Fluorescence* NIH 3T3 Cells Oxidation-Reduction Oxygen / chemistry Photobleaching Video Recording |
| IF | 12.587 |
| Times Cited | 22 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 34 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HeLa(RCB0007) |