論文 - 詳細
| RRC ID | 52682 |
|---|---|
| 著者 | Islam MS, Kaji N, Mikawa S, Yang Q, Kusabe M, Hori M, Ozaki H. |
| タイトル | Induction of myosin light chain kinase and CPI-17 by TGF-β accelerates contractile activity in intestinal epithelial cells. |
| ジャーナル | J Vet Med Sci |
| Abstract |
Epithelial-mesenchymal transition (EMT) is an orchestral and functional change in epithelial cells. Many signaling pathways are involved in EMT, and transforming growth factor-beta (TGF-β) is considered to be one of the most important factors in induction of EMT. In this study, we treated the rat intestinal epithelial cell line (IEC-6) with TGF-β1 as a signaling stimulant. Gross analysis of IEC-6 cells showed typical characteristics of epithelial cells such as cuboidal morphology and cell-cell contact, whereas treatment with TGF-β1 (10 ng/ml-1) for 7 days produced robust, spindle-shaped morphology. Immunocytochemistry analysis showed distinct E-cadherin staining in IEC-6 cells, but weak and faint in EMT cells. EMT cells showed positive expression of α-SMA and tenascin-C but IEC-6 cells did not. Quantitative real-time PCR analysis showed that myosin light chain kinase and C-kinase potentiated protein phosphatase-1 inhibitor (CPI-17) mRNAs were significantly upregulated in EMT cells. Immunocytochemistry analysis also showed that EMT cells strongly expressed CPI-17 but IEC-6 cells did not. A collagen gel contraction assay revealed that EMT cells had greatly increased contraction compared with control cells. These results suggest that the increased contractile activity induced by TGF-β in EMT cells may be attributable to the upregulation of molecules responsible for myosin phosphorylation/de-phosphorylation. |
| 巻・号 | 80(6) |
| ページ | 977-984 |
| 公開日 | 2018-6-29 |
| DOI | 10.1292/jvms.17-0684 |
| PMID | 29695674 |
| PMC | PMC6021892 |
| MeSH | Animals Cyclopropanes / metabolism* Epithelial Cells Epithelial-Mesenchymal Transition* Indoles / metabolism* Japan Myosin-Light-Chain Kinase / metabolism* Rats Transforming Growth Factor beta1 / pharmacology* |
| IF | 0.803 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | IEC 6(RCB0993) |