RRC ID 52688
著者 Emam SE, Ando H, Abu Lila AS, Shimizu T, Ukawa M, Okuhira K, Ishima Y, Mahdy MA, Ghazy FS, Ishida T.
タイトル A Novel Strategy to Increase the Yield of Exosomes (Extracellular Vesicles) for an Expansion of Basic Research.
ジャーナル Biol Pharm Bull
Abstract Exosomes are tiny extracellular vesicles that are usually harvested in small quantities. Such small yield has been an obstacle for the expansion of the basic research regarding exosome analysis and applications in drug delivery. To increase exosome yield, we attempted to stimulate tumor cells via the addition of liposomes in vitro. Neutral, cationic-bare or PEGylated liposomes were incubated with four different tumor cell lines. The stimulatory effect of liposomal formulations on exosome secretion and cellular uptake propensity of the collected exosome by mother cells or different cells was evaluated. Both neutral and cationic-bare liposomes enhanced exosome secretion in a dose-dependent manner. Fluid cationic liposomes provided the strongest stimulation. Surprisingly, the PEGylation of bare liposomes diminished exosome secretion. Exosomes harvested in the presence of fluid cationic liposomes showed increased cellular uptake, but solid cationic liposomes did not. Our findings indicate that the physicochemical properties of liposomes determine whether they will act as a stimulant or as a depressant on exosome secretion from tumor cells. Liposomal stimulation may be a useful strategy to increase exosome yield, although further preparation to increase the purity of exosomes may be needed. In addition, fine-tuning of the biological properties of induced exosomes could be achieved via controlling the physicochemical properties of the stimulant liposomes.
巻・号 41(5)
ページ 733-742
公開日 2018-1-1
DOI 10.1248/bpb.b17-00919
PMID 29709910
MeSH Animals Cell Line, Tumor Exosomes / drug effects* Humans Liposomes / pharmacology* Mice
IF 1.863
引用数 11
リソース情報
ヒト・動物細胞 Colon-26(RCB2657) B16/BL6(RCB2638) MKN45(RCB1001)