論文 - 詳細
| RRC ID | 52696 |
|---|---|
| 著者 | Tsuchida A, Senda M, Ito A, Saito S, Kiso M, Ando T, Harduin-Lepers A, Matsuda A, Furukawa K, Furukawa K. |
| タイトル | Roles of GalNAc-disialyl Lactotetraosyl Antigens in Renal Cancer Cells. |
| ジャーナル | Sci Rep |
| Abstract |
GalNAc-disialyl Lc4 (GalNAc-DSLc4) was reported as a novel antigen that associated with malignant features of renal cell cancers (RCCs). To clarify roles of GalNAc-DSLc4 in malignant properties of RCCs, we identified B4GalNAc-T2 as a responsible gene for the synthesis of GalNAc-DSLc4, and prepared stable transfectants of GalNAc-T2 cDNA using VMRC-RCW cells, resulting in the establishment of high expressants of GalNAc-DSLc4. They showed increased proliferation and invasion, and specific adhesion to laminin. In the transfectants, PI3K/Akt signals were highly activated by serum stimulation or adhesion to laminin. GalNAc-DSLc4 was co-localized in lipid rafts with integrin β1 and caveolin-1 in both immunoblotting of fractionated detergent extracts and immunocytostaining, particularly when stimulated with serum. Masking of GalNAc-DSLc4 with antibodies as well as PI3K inhibitor suppressed malignant properties of the transfectants. These results suggested that GalNAc-DSLc4 is involved in malignant properties of RCCs by forming a molecular complex with integrins in lipid rafts. |
| 巻・号 | 8(1) |
| ページ | 7017 |
| 公開日 | 2018-5-4 |
| DOI | 10.1038/s41598-018-25521-6 |
| PII | 10.1038/s41598-018-25521-6 |
| PMID | 29728594 |
| PMC | PMC5935701 |
| MeSH | Antigens, Neoplasm / immunology* Carcinoma, Renal Cell / immunology* Carcinoma, Renal Cell / metabolism Cell Adhesion Cell Line, Tumor Cell Movement Cell Proliferation Glycolipids Humans Integrins / metabolism Kidney Neoplasms / immunology* Kidney Neoplasms / metabolism Phosphorylation Protein Transport Proto-Oncogene Proteins c-akt / metabolism Signal Transduction |
| IF | 3.998 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | OS-RC-2(RCB0735) RCC10RGB(RCB1151) TUHR4TKB(RCB1198) |