| RRC ID |
52707
|
| 著者 |
Mori J, Sanoh S, Kashiwagi K, Hanada H, Shigeta M, Suzuki KT, Yamamoto T, Kotake Y, Sugihara K, Kitamura S, Kashiwagi A, Ohta S.
|
| タイトル |
Developmental changes in drug-metabolizing enzyme expression during metamorphosis of Xenopus tropicalis.
|
| ジャーナル |
J Toxicol Sci
|
| Abstract |
A large number of chemicals are routinely detected in aquatic environments, and these chemicals may adversely affect aquatic organisms. Accurate risk assessment requires understanding drug-metabolizing systems in aquatic organisms because metabolism of these chemicals is a critical determinant of chemical bioaccumulation and related toxicity. In this study, we evaluated mRNA expression levels of nuclear receptors and drug-metabolizing enzymes as well as cytochrome P450 (CYP) activities in pro-metamorphic tadpoles, froglets, and adult frogs to determine how drug-metabolizing systems are altered at different life stages. We found that drug-metabolizing systems in tadpoles were entirely immature, and therefore, tadpoles appeared to be more susceptible to chemicals compared with metamorphosed frogs. On the other hand, cyp1a mRNA expression and CYP1A-like activity were higher in tadpoles. We found that thyroid hormone (TH), which increases during metamorphosis, induced CYP1A-like activity. Because endogenous TH concentration is significantly increased during metamorphosis, endogenous TH would induce CYP1A-like activity in tadpoles.
|
| 巻・号 |
42(5)
|
| ページ |
605-613
|
| 公開日 |
2017-1-1
|
| DOI |
10.2131/jts.42.605
|
| PMID |
28904296
|
| MeSH |
Animals
Cytochrome P-450 CYP1A1 / genetics
Cytochrome P-450 CYP1A1 / metabolism
Cytochrome P-450 Enzyme System / genetics*
Cytochrome P-450 Enzyme System / metabolism*
Gene Expression / genetics*
Gene Expression Regulation, Developmental / genetics*
Metamorphosis, Biological / genetics*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Receptors, Cytoplasmic and Nuclear / genetics
Receptors, Cytoplasmic and Nuclear / metabolism
Thyroid Hormones / metabolism*
Thyroid Hormones / physiology*
Xenopus / genetics*
Xenopus / physiology*
|
| IF |
1.737
|
| 引用数 |
1
|
| リソース情報 |
| ツメガエル・イモリ |
Xenopus tropicalis |
