論文 - 詳細
| RRC ID | 52707 |
|---|---|
| 著者 | Mori J, Sanoh S, Kashiwagi K, Hanada H, Shigeta M, Suzuki KT, Yamamoto T, Kotake Y, Sugihara K, Kitamura S, Kashiwagi A, Ohta S. |
| タイトル | Developmental changes in drug-metabolizing enzyme expression during metamorphosis of Xenopus tropicalis. |
| ジャーナル | J Toxicol Sci |
| Abstract |
A large number of chemicals are routinely detected in aquatic environments, and these chemicals may adversely affect aquatic organisms. Accurate risk assessment requires understanding drug-metabolizing systems in aquatic organisms because metabolism of these chemicals is a critical determinant of chemical bioaccumulation and related toxicity. In this study, we evaluated mRNA expression levels of nuclear receptors and drug-metabolizing enzymes as well as cytochrome P450 (CYP) activities in pro-metamorphic tadpoles, froglets, and adult frogs to determine how drug-metabolizing systems are altered at different life stages. We found that drug-metabolizing systems in tadpoles were entirely immature, and therefore, tadpoles appeared to be more susceptible to chemicals compared with metamorphosed frogs. On the other hand, cyp1a mRNA expression and CYP1A-like activity were higher in tadpoles. We found that thyroid hormone (TH), which increases during metamorphosis, induced CYP1A-like activity. Because endogenous TH concentration is significantly increased during metamorphosis, endogenous TH would induce CYP1A-like activity in tadpoles. |
| 巻・号 | 42(5) |
| ページ | 605-613 |
| 公開日 | 2017-1-1 |
| DOI | 10.2131/jts.42.605 |
| PMID | 28904296 |
| MeSH | Animals Cytochrome P-450 CYP1A1 / genetics Cytochrome P-450 CYP1A1 / metabolism Cytochrome P-450 Enzyme System / genetics* Cytochrome P-450 Enzyme System / metabolism* Gene Expression / genetics* Gene Expression Regulation, Developmental / genetics* Metamorphosis, Biological / genetics* RNA, Messenger / genetics RNA, Messenger / metabolism Receptors, Cytoplasmic and Nuclear / genetics Receptors, Cytoplasmic and Nuclear / metabolism Thyroid Hormones / metabolism* Thyroid Hormones / physiology* Xenopus / genetics* Xenopus / physiology* |
| IF | 1.737 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ツメガエル・イモリ | Xenopus tropicalis |