論文 - 詳細
| RRC ID | 52745 |
|---|---|
| 著者 | Ogawa H, Fujikura D, Ohnuma M, Ohnishi N, Hang'ombe BM, Mimuro H, Ezaki T, Mweene AS, Higashi H. |
| タイトル | A novel multiplex PCR discriminates Bacillus anthracis and its genetically related strains from other Bacillus cereus group species. |
| ジャーナル | PLoS One |
| Abstract |
Anthrax is an important zoonotic disease worldwide that is caused by Bacillus anthracis, a spore-forming pathogenic bacterium. A rapid and sensitive method to detect B. anthracis is important for anthrax risk management and control in animal cases to address public health issues. However, it has recently become difficult to identify B. anthracis by using previously reported molecular-based methods because of the emergence of B. cereus, which causes severe extra-intestinal infection, as well as the human pathogenic B. thuringiensis, both of which are genetically related to B. anthracis. The close genetic relation of chromosomal backgrounds has led to complexity of molecular-based diagnosis. In this study, we established a B. anthracis multiplex PCR that can screen for the presence of B. anthracis virulent plasmids and differentiate B. anthracis and its genetically related strains from other B. cereus group species. Six sets of primers targeting a chromosome of B. anthracis and B. anthracis-like strains, two virulent plasmids, pXO1 and pXO2, a bacterial gene, 16S rRNA gene, and a mammalian gene, actin-beta gene, were designed. The multiplex PCR detected approximately 3.0 CFU of B. anthracis DNA per PCR reaction and was sensitive to B. anthracis. The internal control primers also detected all bacterial and mammalian DNAs examined, indicating the practical applicability of this assay as it enables monitoring of appropriate amplification. The assay was also applied for detection of clinical strains genetically related to B. anthracis, which were B. cereus strains isolated from outbreaks of hospital infections in Japan, and field strains isolated in Zambia, and the assay differentiated B. anthracis and its genetically related strains from other B. cereus group strains. Taken together, the results indicate that the newly developed multiplex PCR is a sensitive and practical method for detecting B. anthracis. |
| 巻・号 | 10(3) |
| ページ | e0122004 |
| 公開日 | 2015-1-1 |
| DOI | 10.1371/journal.pone.0122004 |
| PII | PONE-D-14-52927 |
| PMID | 25774512 |
| PMC | PMC4361551 |
| MeSH | Animal Diseases / diagnosis Animal Diseases / microbiology Animals Anthrax / diagnosis Anthrax / microbiology Bacillus anthracis / classification Bacillus anthracis / genetics* Bacillus cereus / classification Bacillus cereus / genetics* Chromosomes, Bacterial Gram-Positive Bacterial Infections / diagnosis Gram-Positive Bacterial Infections / microbiology Humans Multiplex Polymerase Chain Reaction* Plasmids / genetics RNA, Bacterial RNA, Ribosomal, 16S / genetics Sensitivity and Specificity |
| IF | 2.776 |
| 引用数 | 10 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原微生物 | 病原細菌 |