RRC ID 52749
Author Narusaka M, Iuchi S, Narusaka Y.
Title Analyses of natural variation indicates that the absence of RPS4/RRS1 and amino acid change in RPS4 cause loss of their functions and resistance to pathogens.
Journal Plant Signal Behav
Abstract A pair of Arabidopsis thaliana resistance proteins, RPS4 and RRS1, recognizes the cognate Avr effector from the bacterial pathogens Pseudomonas syringae pv. tomato expressing avrRps4 (Pst-avrRps4), Ralstonia solanacearum, and the fungal pathogen Colletotrichum higginsianum and leads to defense signaling activation against the pathogens. In the present study, we analyzed 14 A. thaliana accessions for natural variation in Pst-avrRps4 and C. higginsianum susceptibility, and found new compatible and incompatible Arabidopsis-pathogen interactions. We first found that A. thaliana accession Cvi-0 is susceptible to Pst-avrRps4. Interestingly, the genome sequence assembly indicated that Cvi-0 lost both RPS4 and RRS1, but not RPS4B and RRS1B, compared to the reference genome sequence from A. thaliana accession Col-0. On the other hand, the natural variation analysis of RPS4 alleles from various Arabidopsis accessions revealed that one amino-acid change, Y950H, is responsible for the loss of resistance to Pst-avrRps4 and C. higginsianum in RLD-0. Our data indicate that the amino acid change, Y950H, in RPS4 resulted in the loss of both RPS4 and RRS1 functions and resistance to pathogens.
Volume 12(3)
Pages e1293218
Published 2017-3-4
DOI 10.1080/15592324.2017.1293218
PMID 28277970
PMC PMC5399910
MeSH Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis / microbiology* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Colletotrichum / pathogenicity Gene Expression Regulation, Plant Plant Proteins / genetics Plant Proteins / metabolism* Pseudomonas syringae / pathogenicity Ralstonia solanacearum / pathogenicity Transcription Factors / genetics Transcription Factors / metabolism
IF 1.671
Times Cited 2
Arabidopsis / Cultured plant cells, genes sja05800 sjw14100 sja25200 sjw12700