RRC ID 52751
Author Mano J, Ishibashi A, Muneuchi H, Morita C, Sakai H, Biswas MS, Koeduka T, Kitajima S.
Title Acrolein-detoxifying isozymes of glutathione transferase in plants.
Journal Planta
Abstract MAIN CONCLUSION:Acrolein is a lipid-derived highly reactive aldehyde, mediating oxidative signal and damage in plants. We found acrolein-scavenging glutathione transferase activity in plants and purified a low K M isozyme from spinach. Various environmental stressors on plants cause the generation of acrolein, a highly toxic aldehyde produced from lipid peroxides, via the promotion of the formation of reactive oxygen species, which oxidize membrane lipids. In mammals, acrolein is scavenged by glutathione transferase (GST; EC 2.5.1.18) isozymes of Alpha, Pi, and Mu classes, but plants lack these GST classes. We detected the acrolein-scavenging GST activity in four species of plants, and purified an isozyme showing this activity from spinach (Spinacia oleracea L.) leaves. The isozyme (GST-Acr), obtained after an affinity chromatography and two ion exchange chromatography steps, showed the K M value for acrolein 93 μM, the smallest value known for acrolein-detoxifying enzymes in plants. Peptide sequence homology search revealed that GST-Acr belongs to the GST Tau, a plant-specific class. The Arabidopsis thaliana GST Tau19, which has the closest sequence similar to spinach GST-Acr, also showed a high catalytic efficiency for acrolein. These results suggest that GST plays as a scavenger for acrolein in plants.
Volume 245(2)
Pages 255-264
Published 2017-2
DOI 10.1007/s00425-016-2604-5
PII 10.1007/s00425-016-2604-5
PMID 27718072
MeSH Acrolein / metabolism* Arabidopsis Proteins / chemistry Arabidopsis Proteins / metabolism Chromatography, Affinity Chromatography, Ion Exchange Glutathione Transferase / chemistry Glutathione Transferase / genetics Glutathione Transferase / isolation & purification Glutathione Transferase / metabolism* Inactivation, Metabolic Isoenzymes / genetics Isoenzymes / isolation & purification Isoenzymes / metabolism Plant Leaves / enzymology Plant Proteins / isolation & purification Plant Proteins / metabolism* Sequence Homology, Amino Acid Spinacia oleracea / enzymology*
IF 3.249
Resource
Arabidopsis / Cultured plant cells, genes pda00742