論文 - 詳細
| RRC ID | 52759 |
|---|---|
| 著者 | Fukudome M, Calvo-Begueria L, Kado T, Osuki K, Rubio MC, Murakami E, Nagata M, Kucho K, Sandal N, Stougaard J, Becana M, Uchiumi T. |
| タイトル | Hemoglobin LjGlb1-1 is involved in nodulation and regulates the level of nitric oxide in the Lotus japonicus-Mesorhizobium loti symbiosis. |
| ジャーナル | J Exp Bot |
| Abstract |
Leghemoglobins transport and deliver O2 to the symbiosomes inside legume nodules and are essential for nitrogen fixation. However, the roles of other hemoglobins (Hbs) in the rhizobia-legume symbiosis are unclear. Several Lotus japonicus mutants affecting LjGlb1-1, a non-symbiotic class 1 Hb, have been used to study the function of this protein in symbiosis. Two TILLING alleles with single amino acid substitutions (A102V and E127K) and a LORE1 null allele with a retrotransposon insertion in the 5'-untranslated region (96642) were selected for phenotyping nodulation. Plants of all three mutant lines showed a decrease in long infection threads and nodules, and an increase in incipient infection threads. About 4h after inoculation, the roots of mutant plants exhibited a greater transient accumulation of nitric oxide (NO) than did the wild-type roots; nevertheless, in vitro NO dioxygenase activities of the wild-type, A102V, and E127K proteins were similar, suggesting that the mutated proteins are not fully functional in vivo The expression of LjGlb1-1, but not of the other class 1 Hb of L. japonicus (LjGlb1-2), was affected during infection of wild-type roots, further supporting a specific role for LjGlb1-1. In conclusion, the LjGlb1-1 mutants reveal that this protein is required during rhizobial infection and regulates NO levels. |
| 巻・号 | 67(17) |
| ページ | 5275-83 |
| 公開日 | 2016-9-1 |
| DOI | 10.1093/jxb/erw290 |
| PII | erw290 |
| PMID | 27443280 |
| PMC | PMC5014168 |
| MeSH | Hemoglobins / metabolism Hemoglobins / physiology* Lotus / growth & development Lotus / metabolism Lotus / microbiology Lotus / physiology* Mesorhizobium / metabolism Mesorhizobium / physiology* Nitric Oxide / metabolism* Plant Proteins / metabolism Plant Proteins / physiology* Plant Root Nodulation / physiology* Plant Roots / growth & development Plant Roots / microbiology Plant Roots / physiology Real-Time Polymerase Chain Reaction Symbiosis / physiology |
| IF | 5.908 |
| 引用数 | 16 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ミヤコグサ・ダイズ | Gifu B-129 |