論文 - 詳細
| RRC ID | 52785 |
|---|---|
| 著者 | Toyotome T, Hagiwara D, Watanabe A, Kamei K. |
| タイトル | A simple method to detect the tandem repeat of the cyp51A promoter in azole-resistant strains of Aspergillus fumigatus. |
| ジャーナル | Med Mycol |
| Abstract |
We designed primers and cycling probes to detect the tandem repeat (TR) of cyp51A promoter in Aspergillus fumigatus. A control-probe was designed to anneal to the outside of the TR region, whereas a TR-probe was designed to anneal to the inside of the TR region. For amplification and probe-hydrolysis detection, the CycleavePCR system was used. Although the difference between Ct values of the wild-type genome for the control-probe and the TR-probe was around -0.1, the difference between Ct values of TR-harboring strains was around 0.7. These data indicate that this is a simple method to detect TR in azole-resistant A. fumigatus. |
| 巻・号 | 56(8) |
| ページ | 1042-1044 |
| 公開日 | 2018-11-1 |
| DOI | 10.1093/mmy/myx149 |
| PII | 4801109 |
| PMID | 29340615 |
| MeSH | Antifungal Agents / pharmacology* Aspergillus fumigatus / drug effects* Aspergillus fumigatus / genetics Azoles / pharmacology* Cytochrome P-450 Enzyme System / genetics* Fungal Proteins / genetics* Hydrolysis Microbiological Techniques / methods Nucleic Acid Amplification Techniques / methods* Oligonucleotide Probes Promoter Regions, Genetic* Tandem Repeat Sequences* |
| IF | 2.822 |
| 引用数 | 3 |
| リソース情報 | |
| 病原真核微生物 | 病原真菌: IFM 63432 |