RRC ID 52794
Author de Souza M, Matsuzawa T, Sakai K, Muraosa Y, Lyra L, Busso-Lopes AF, Levin ASS, Schreiber AZ, Mikami Y, Gonoi T, Kamei K, Moretti ML, Trabasso P.
Title Comparison of DNA Microarray, Loop-Mediated Isothermal Amplification (LAMP) and Real-Time PCR with DNA Sequencing for Identification of Fusarium spp. Obtained from Patients with Hematologic Malignancies.
Journal Mycopathologia
Abstract The performance of three molecular biology techniques, i.e., DNA microarray, loop-mediated isothermal amplification (LAMP), and real-time PCR were compared with DNA sequencing for properly identification of 20 isolates of Fusarium spp. obtained from blood stream as etiologic agent of invasive infections in patients with hematologic malignancies. DNA microarray, LAMP and real-time PCR identified 16 (80%) out of 20 samples as Fusarium solani species complex (FSSC) and four (20%) as Fusarium spp. The agreement among the techniques was 100%. LAMP exhibited 100% specificity, while DNA microarray, LAMP and real-time PCR showed 100% sensitivity. The three techniques had 100% agreement with DNA sequencing. Sixteen isolates were identified as FSSC by sequencing, being five Fusarium keratoplasticum, nine Fusarium petroliphilum and two Fusarium solani. On the other hand, sequencing identified four isolates as Fusarium non-solani species complex (FNSSC), being three isolates as Fusarium napiforme and one isolate as Fusarium oxysporum. Finally, LAMP proved to be faster and more accessible than DNA microarray and real-time PCR, since it does not require a thermocycler. Therefore, LAMP signalizes as emerging and promising methodology to be used in routine identification of Fusarium spp. among cases of invasive fungal infections.
Volume 182(7-8)
Pages 625-632
Published 2017-8
DOI 10.1007/s11046-017-0129-5
PII 10.1007/s11046-017-0129-5
PMID 28324245
IF 1.476
Resource
Pathogenic eukaryotic microorganisms 病原真菌: IFM 4823 IFM 4827 IFM 4852 IFM 4853 IFM 4857 IFM 4874 IFM 4878 IFM 4879 IFM 4880 IFM 4957 IFM 5270 IFM 5275 IFM 5277 IFM 5316 IFM 5322 IFM 5397 IFM 5446 IFM 5462 IFM 5473 IFM 5476 IFM 5520 IFM 5527 IFM 5758 IFM 5760 IFM 5761 IFM 5763 IFM 5766 IFM 5768 IFM 5774 IFM 5775 IFM 5788 IFM 40051 IFM 40056 IFM 40059 IFM 40062 IFM 40064 IFM 40079 IFM 40131 IFM 40501 IFM 40506 IFM 40508 IFM 40515 IFM 40517 IFM 40627 IFM 40707 IFM 40718 IFM 40741 IFM 40753 IFM 40777 IFM 40784 IFM 40785 IFM 41145 IFM 41147 IFM 41172 IFM 41259 IFM 41310 IFM 41311 IFM 41317 IFM 41330 IFM 41345 IFM 41346 IFM 41395 IFM 41485 IFM 41500 IFM 41504 IFM 41505 IFM 41560 IFM 41561 IFM 41582 IFM 41584 IFM 41586 IFM 41587 IFM 41591 IFM 41592 IFM 41593 IFM 41594 IFM 41620 IFM 41948 IFM 45809 IFM 45822 IFM 45868 IFM 46007 IFM 46012 IFM 46109 IFM 46110 IFM 46921 IFM 47048 IFM 47052 IFM 47053 IFM 47054 IFM 47055 IFM 47056 IFM 47183 IFM 47207 IFM 47279 IFM 47302 IFM 47464 IFM 47468 IFM 47490 IFM 47649 IFM 47650 IFM 47651 IFM 47652 IFM 47656 IFM 47659 IFM 47743 IFM 47751 IFM 47752 IFM 47757 IFM 47764 IFM 47796 IFM 47882 IFM 47971 IFM 47973 IFM 48036 IFM 48054 IFM 48162 IFM 48189 IFM 48212 IFM 48213 IFM 48313 IFM 48361 IFM 48392 IFM 48429 IFM 48434 IFM 48440 IFM 48449 IFM 48456 IFM 48461 IFM 48494 IFM 48500 IFM 48501 IFM 48515 IFM 48516 IFM 48517 IFM 48518 IFM 48529 IFM 48530 IFM 48531 IFM 48537 IFM 48538 IFM 48539 IFM 48551 IFM 48558 IFM 48559 IFM 48560 IFM 48563 IFM 48585 IFM 48587 IFM 48588 IFM 48589 IFM 48592 IFM 48611 IFM 48617 IFM 48620 IFM 48625 IFM 48648 IFM 48675 IFM 48794 IFM 48820 IFM 49195 IFM 49204 IFM 49205 IFM 49207 IFM 49209 IFM 49210 IFM 49387 IFM 49447 IFM 49593 IFM 49595 IFM 49674 IFM 49678 IFM 49800 IFM 49801 IFM 49802 IFM 49803 IFM 49804 IFM 49805 IFM 49806 IFM 49810 IFM 49812 IFM 49813 IFM 49814 IFM 49816 IFM 49818 IFM 49819 IFM 49820 IFM 49821 IFM 49822 IFM 49888 IFM 49907 IFM 49911 IFM 50017 IFM 50233 IFM 50467 IFM 50485 IFM 50680 IFM 50887 IFM 50912 IFM 50994 IFM 51070 IFM 51110 IFM 51144 IFM 51356 IFM 51387 IFM 51426 IFM 52151 IFM 52211 IFM 52212 IFM 52350 IFM 52628 IFM 52635 IFM 52637 IFM 52679 IFM 52680 IFM 52682 IFM 52867 IFM 52915 IFM 52933 IFM 52999 IFM 53022 IFM 53163 IFM 53465 IFM 53506 IFM 53856 IFM 54120 IFM 54153 IFM 54229 IFM 54250 IFM 54624 IFM 54794 IFM 54891 IFM 55023 IFM 55024 IFM 55168 IFM 55169 IFM 55217 IFM 55413 IFM 55414 IFM 55446 IFM 55589 IFM 55890 IFM 55928 IFM 56021 IFM 56042 IFM 56062 IFM 56082 IFM 56102 IFM 56171 IFM 56172 IFM 56188 IFM 56195 IFM 56200 IFM 56204 IFM 56209 IFM 56212 IFM 56213 IFM 56214 IFM 56215 IFM 56218 IFM 56222 IFM 56223 IFM 56225 IFM 56228 IFM 56239 IFM 56365 IFM 56393 IFM 56400 IFM 56868 IFM 56909 IFM 56936 IFM 56985 IFM 56987 IFM 56988 IFM 57012 IFM 57066 IFM 57067 IFM 57161 IFM 57235 IFM 57239 IFM 57249 IFM 57250 IFM 57251 IFM 57298 IFM 57300 IFM 57323 IFM 57371 IFM 57469 IFM 57482 IFM 57491 IFM 57500 IFM 57501 IFM 57663 IFM 57730 IFM 58002 IFM 58035 IFM 58094 IFM 58095 IFM 58100 IFM 58115 IFM 58692 IFM 58948 IFM 59016 IFM 59169 IFM 59178 IFM 59336 IFM 59484 IFM 59501 IFM 59549 IFM 59622 IFM 59638 IFM 59830 IFM 59943 IFM 59955 IFM 60091 IFM 60108 IFM 60194 IFM 60240 IFM 60287 IFM 60307 IFM 60448 IFM 60471 IFM 60472 IFM 60605 IFM 60655 IFM 60656 IFM 60662 IFM 60677 IFM 60678 IFM 60684 IFM 60797 IFM 60829 IFM 60909 IFM 60912 IFM 60916 IFM 60928 IFM 60996 IFM 61222 IFM 61229 IFM 61254 IFM 61270 IFM 61275 IFM 61282 IFM 61321 IFM 61373 IFM 61389 IFM 61452 IFM 61455 IFM 61523 IFM 61597 IFM 61612 IFM 61613 IFM 61624 IFM 61759 IFM 61863