Reference - Detail
| RRC ID | 52795 |
|---|---|
| Author | Yamaguchi M, Takahashi-Nakaguchi A, Aida Y, Sato-Okamoto M, Chibana H. |
| Title | Convenient method for better preservation of fine structures of cultured macrophages and engulfed yeast cells by freeze-substitution fixation. |
| Journal | Microscopy (Oxf) |
| Abstract |
Rapid freeze-freeze substitution after glutaraldehyde fixation (CF-FS method) obtained the natural and fine structures of macrophages and engulfed yeast cells. Culturing macrophages on single hole molybdenum grids placed in culture dishes made possible the rapid freezing of cells by the 'open sandwich method'. This method may be convenient when rapid-freezing cannot be performed immediately, or when a rapid-freezing device is not available in the lab. |
| Volume | 66(3) |
| Pages | 209-211 |
| Published | 2017-6-1 |
| DOI | 10.1093/jmicro/dfx006 |
| PII | 3003170 |
| PMID | 28339650 |
| MeSH | Animals Candida / ultrastructure* Cell Line Cryopreservation / methods* Fixatives Freeze Substitution / methods* Glutaral Macrophages / cytology* Macrophages / microbiology Macrophages / physiology Macrophages / ultrastructure Mice Microscopy, Electron, Transmission Phagocytosis Saccharomyces cerevisiae / ultrastructure* Tissue Fixation / methods* |
| IF | 1.394 |
| Times Cited | 1 |
| Resource | |
| Pathogenic eukaryotic microorganisms | 病原真菌：IFM 63009, IFM 60665, IFM 5768 |