RRC ID 52799
Author Kusuya Y, Hagiwara D, Sakai K, Yaguchi T, Gonoi T, Takahashi H.
Title Transcription factor Afmac1 controls copper import machinery in Aspergillus fumigatus.
Journal Curr Genet
Abstract Copper (Cu) is an essential metal for all living organisms, although it is toxic in excess. Filamentous fungus must acquire copper from its environment for growth. Despite its essentiality for growth, the mechanisms that maintain copper homeostasis are not fully understood in filamentous fungus. To gain insights into copper homeostasis, we investigated the roles of a copper transcription factor Afmac1 in the life-threatening fungus Aspergillus fumigatus, a homolog of the yeast MAC1. We observed that the Afmac1 deletion mutant exhibited not only significantly slower growth, but also incomplete conidiation including a short chain of conidia and defective melanin. Moreover, the expressions of the copper transporters, ctrA1, ctrA2, and ctrC, and metalloreductases, Afu8g01310 and fre7, were repressed in ∆Afmac1 cells, while those expressions were induced under copper depletion conditions in wild-type. The expressions of pksP and wetA, which are, respectively, involved in biosynthesis of conidia-specific melanin and the late stage of conidiogenesis, were decreased in the ∆Afmac1 strain under minimal media condition. Taken together, these results indicate that copper acquisition through AfMac1 functions in growth as well as conidiation.
Volume 63(4)
Pages 777-789
Published 2017-8-1
DOI 10.1007/s00294-017-0681-z
PII 10.1007/s00294-017-0681-z
PMID 28215034
MeSH Aspergillus fumigatus / genetics* Aspergillus fumigatus / metabolism Copper / metabolism* Fungal Proteins / genetics* Gene Expression Regulation, Fungal Homeostasis Melanins / genetics Melanins / metabolism Nuclear Proteins / genetics Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae Proteins / genetics Spores, Fungal / genetics Spores, Fungal / metabolism Transcription Factors / genetics*
IF 3.464
Times Cited 13
Pathogenic eukaryotic microorganisms IFM 54229 (= Af293)