論文 - 詳細
| RRC ID | 52820 |
|---|---|
| 著者 | Haraguchi T, Tominaga M, Nakano A, Yamamoto K, Ito K. |
| タイトル | Myosin XI-I is Mechanically and Enzymatically Unique Among Class-XI Myosins in Arabidopsis. |
| ジャーナル | Plant Cell Physiol |
| Abstract |
Arabidopsis possesses 13 genes encoding class-XI myosins. Among these, myosin XI-I is phylogenetically distant. To examine the molecular properties of Arabidopsis thaliana myosin XI-I (At myosin XI-I), we performed in vitro mechanical and enzymatic analyses using recombinant constructs of At myosin XI-I. Unlike other biochemically studied class-XI myosins, At myosin XI-I showed extremely low actin-activated ATPase activity (Vmax = 3.7 Pi s(-1) head(-1)). The actin-sliding velocity of At myosin XI-I was 0.25 µm s(-1), >10 times lower than those of other class-XI myosins. The ADP dissociation rate from acto-At myosin XI-I was 17 s(-1), accounting for the low actin-sliding velocity. In contrast, the apparent affinity for actin in the presence of ATP, estimated from Kapp (0.61 µM) of actin-activated ATPase, was extremely high. The equilibrium dissociation constant for actin was very low in both the presence and absence of ATP, indicating a high affinity for actin. To examine At myosin XI-I motility in vivo, green fluorescent protein-fused full-length At myosin XI-I was expressed in cultured Arabidopsis cells. At myosin XI-I localized not only on the nuclear envelope but also on small dots moving slowly (0.23 µm s(-1)) along actin filaments. Our results show that the properties of At myosin XI-I differ from those of other Arabidopsis class-XI myosins. The data suggest that At myosin XI-I does not function as a driving force for cytoplasmic streaming but regulates the organelle velocity, supports processive organelle movement or acts as a tension generator. |
| 巻・号 | 57(8) |
| ページ | 1732-43 |
| 公開日 | 2016-8-1 |
| DOI | 10.1093/pcp/pcw097 |
| PII | pcw097 |
| PMID | 27273580 |
| MeSH | Actin Cytoskeleton / metabolism Actins / metabolism Adenosine Triphosphate / metabolism Arabidopsis / cytology Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cell Nucleus / metabolism Cytoplasmic Streaming Genes, Reporter Molecular Motor Proteins / genetics Molecular Motor Proteins / metabolism* Organelles / metabolism Protein Transport |
| IF | 4.062 |
| 引用数 | 9 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda19579 |