RRC ID 52857
Author Shinkai Y, Takio K, Okumura K.
Title Homology of perforin to the ninth component of complement (C9).
Journal Nature
Abstract Perforin is one of the cytolytic factors present in the cytoplasmic granules of mouse cytotoxic T lymphocytes and natural killer cells. We have determined the sequence of the N-terminal amino acids of perforin purified from a mouse natural killer cell line, and, by using oligonucleotide probes corresponding to the amino acid residues, we have identified a complementary DNA encoding perforin from the cDNA library of a mouse cytotoxic T lymphocyte clone. As predicted from the functional similarities between perforin and the ninth component of the serum cytolytic system, complement (C9) (refs 4-8), the deduced primary structure of perforin has homology with C9 at their respective functionally conserved regions. We find that perforin is only expressed in killer cell lines, and not in helper T lymphocytes or other tumour cells tested. Thus we have provided direct molecular evidence that a killer-cell-specific protein evolutionally linked to C9 is involved in cell-mediated cytolysis.
Volume 334(6182)
Pages 525-7
Published 1988-8-11
DOI 10.1038/334525a0
PMID 3261391
MeSH Amino Acid Sequence Animals Base Sequence* Complement C9* / genetics DNA / genetics Humans Membrane Glycoproteins* Membrane Proteins* / genetics Mice Molecular Sequence Data Molecular Weight Perforin Pore Forming Cytotoxic Proteins Protein Processing, Post-Translational Sequence Homology, Nucleic Acid* T-Lymphocytes, Cytotoxic / analysis* Trout
IF 43.07
Times Cited 246
Resource
DNA material P-1-1 (RDB03178).